硅钨杂多酸与牛血红蛋白相互作用的研究  

Interaction between Silicotungstic Heteropolyacid and Bovine Hemoglobin (BHb)

作　　者：王彦卿[1] 张红梅[1] 张根成 

机构地区：[1]江苏省滩涂生物资源与环境保护重点建设实验室盐城师范学院应用化学与环境工程研究所,盐城224002

出　　处：《无机化学学报》2006年第5期895-899,共5页Chinese Journal of Inorganic Chemistry

基　　金：江苏省滩涂生物资源与环境保护重点建设实验室资助项目(No.JLCBE05032)

摘　　要：The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a major role in stabilizing the complex. The effect of silicotungstic heteropolyacid on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy.The interaction between silicotungstic heteropolyacid and bovine hemoglobin （BHb） was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant Ks and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a mation of BHb was major role in analyzed stabilizing the complex. The effect of silicotungstic heteropolyacid on the conforusing synchronous fluorescence spectroscopy.

关 键 词：硅钨杂多酸 牛血红蛋白 紫外光谱 荧光光谱 热力学参数 

分 类 号：O614.613[理学—无机化学] O613.72[理学—化学]