In situ observation of C_(60)(C(COOH)_(2))_(2) interacting with living cells using fluorescence microscopy  

作　　者：YE Chang CHEN Chunying CHEN Zhen MENG Huan XING Li JIANG Yaxin YUAN Hui XING Gengmei ZHAO Feng ZHAO Yuliang CHAI Zhifang FANG Xiaohong HAN Dong CHEN Long WANG Chen WEI Taotao 

机构地区：[1]Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials, Institute of High Energy of Physics, Chinese Academy of Sciences, Beijing 100049, China [2]Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China [3]National Center for Nanoscience and Technology, Beijing 100080, China [4]Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Chinese Science Bulletin》2006年第9期1060-1064,共5页

基　　金：This work was supported by the National Natural Science Foundation of China （Grant Nos. 10490180 and 90406024）;the 0utstanding Young Natural Scientist Foundation （Grant Nos. 10525524 and 20225516）; the Ministry of Science and Technology （Grant No. 2005CB724703）;the Directionality Program of the Chinese Academy of Sciences （Grant No. KJCX-SW-H12）.

摘　　要：The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this pur- pose, the C60 derivative C60(C(COOH)2)2, a thera- peutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cy- tometry. The results indicate that C60(C(COOH)2)2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2)2 can carry the molecule that cannot cross cellular membranes into cells, because fluo- rescein compound itself cannot enter the cell or ad- here to membrane. At concentrations ranging from 1×10?2 to 1×102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity.The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C60 derivative C60（C（COOH）2）2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60（C（COOH）2）2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60（C（COOH）2）2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1×10^-2 to 1×10^2 mg/L, C60（C（COOH）2）2 does not show any detectable cytotoxicity.

关 键 词：C60(C(COOH)2)2 原位观察 真实时间成象 球壳状碳分子 细胞膜 荧光标记 

分 类 号：TB383[一般工业技术—材料科学与工程]