Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers  

作　　者：QIN Jun CHEN Weiyuan GUAN Rongxia JIANG Chengxi LI Yinghui FU Yashu LIU Zhangxiong ZHANG Mengchen CHANG Ruzhen QIU Lijuan 

机构地区：[1]National Key Facility of Crop Gene Resources and Genetic Improvement, Key Lab of Crop Gerrnplasm Resources & Biotechology (MOA), Institute of Crop Science, Chinese Agricultural Academy of Sciences, Beijing 100081, China [2]Hebei Academy of Agricultural and Forestry Science, Shijiazhuang 050031, China [3]Heilongjiang Academy of Agricultural Science, Suihua 152052, China

出　　处：《Chinese Science Bulletin》2006年第9期1078-1084,共7页

基　　金：This work was supported by the National Scientific Research Program （Grant No. 2004BA525 B06）;High-Tech Plan （863） （Grant No. 2004AA211111）;the National Basic Research Program （973） （Grant No. 2004 CBl17213）;the National Natural Science Foundation of China （Grant No. 30490250）.

摘　　要：Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25.Simple sequence repeats （SSR） marker analysis, combined with pedigree analysis, was carried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of foreign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean cultivars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25 between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group （LG） I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes： two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus for protein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25.

关 键 词：大豆 谱系分析 SSR 序列分析 种质资源 

分 类 号：S565.1[农业科学—作物学]