纳洛酮对实验性心肺复苏脑氧自由基表达的影响  

Effect of naloxone on the expression of oxygen free radical in dog′s cerebral tissue after experimental cardiopulmonary resuscitation

作　　者：许松青[1] 陈卓堃[2] 张弋[1] 林珮仪[1] 李燕屏[1] 孙明[3] 

机构地区：[1]广州医学院第二附属医院急诊科,510260 [2]广州市第一人民医院急诊科,510180 [3]中南大学附属湘雅医院,长沙市410007

出　　处：《实用医学杂志》2006年第9期990-992,共3页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金资助项目(编号:A2005066)

摘　　要：目的:探讨纳洛酮对心肺复苏后脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)浓度及其形态学的影响。方法:18只健康杂种犬,随机分成3组(n=6)。空白组,不诱发室颤,仅行预处理;对照组,心跳骤停后予常规心肺复苏术;纳洛酮组,心跳骤停后予常规心肺复苏术并使用纳洛酮。空白组预处理后6h、对照组和纳洛酮组复苏后6h取脑组织行MDA、SOD浓度测定以及形态学检查。结果:对照组脑组织MDA的含量高于空白组(P<0.01)和纳洛酮组(P<0.01);而纳洛酮组中脑组织SOD的含量高于对照组(P<0.01),低于空白组(P<0.01)。对照组神经元出现了明显的病理损害,而纳洛酮组神经元的病理损害低于对照组。结论:纳洛酮可减轻心肺复苏后脑组织MDA的生成,增加SOD的活力,并可减轻其病理损害,从而减轻神经元的再灌注损伤。Objective To explore the effect of naloxone on the expression of MDA and SOD in dog＇s cerebral tissue and the morphological change of the tissue after cardiopulmonary resuacitation（CPR）. Methods 18 healthy adult mongrel dogs were randomly divided into three groups （ n = 6）, Blank group： The dogs were not induced ventriculur fibrillation and their cerebral tissues were collected; Control group： The dogs were performed with standard CPR after cardiac arrest; Naloxone group： The dogs were performed with standard CPR and administerod with naloxone after cardiac arrest, The cerebral tissues of the dogs in both control group and naloxone group were collected 6 hours after CPR. The levels of MDA and SOD were detected and the pathological changes of the cerebral tissues were examined. Results The level of MDA in the control group was higher than that in either the blank group （ P 〈 0. 01 ） or the naloxone group （ P 〈 0. 01 ）, while the level of SOD in the Naloxone group was higher than that in the control group （ P 〈 0. 01）, but lower than that in the blank group （ P 〈 0. 01 ）. The neurons of the cerebral tissues from the control group revealed obvious damage and the damage was more severe than that of the neurons from the Naloxone group. Conclusions Naloxone can reduce the development of MDA in the cerebral tissue and increase the activity of SOD after CPR. It may relieve the reperfusion injury of the neurons by reducing the damage of the cerebral tissue.

关 键 词：心肺复苏术 丙二醛 超氧化物歧化酶 纳洛酮 犬 

分 类 号：R459.7[医药卫生—急诊医学]