抗疟治疗对宿主保护性免疫影响的实验研究  

Influence of anti-malarial therapy on the acquirement protective immunity in mice infected in vitro malaria parasite

作　　者：马世红[1] 刘军[1] 刘英杰[2] 冯辉[1] 吴忆[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110001 [2]中国医科大学基础医学院病原生物学教研室

出　　处：《中国人兽共患病学报》2006年第4期338-341,共4页Chinese Journal of Zoonoses

摘　　要：目的探讨药物治疗对宿主保护性免疫建立的影响。方法用不同剂量氯喹或青蒿琥酯治疗约氏疟原虫(非致死型)感染的BALB/c小鼠。待自愈组清除疟原虫后30d,用约氏疟原虫(非致死型和致死型)以及伯氏疟原虫再次感染。以吉姆萨薄血膜染色法观察小鼠虫体血症水平,采用ELISA法检测脾细胞培养上清中IFN-γ水平和血清中特异性抗体水平。结果自愈组和各治疗组小鼠于初次感染早期均产生高水平IFN-γ,随之抗约氏疟原虫(非致死型)特异性IgG抗体水平显著升高,且无明显差异。对约氏疟原虫(非致死型和致死型)再次攻击小鼠均呈完全抵抗,少数出现低水平一过性虫体血症。而异种疟原虫攻击时小鼠全部感染并死亡。结论氯喹或青蒿琥酯不同剂量治疗不影响小鼠保护性免疫的建立和免疫记忆的维持,特异性IgG抗体是宿主抵御再感染主要的免疫效应分子。In order to investigate the influence of anti-malarial therapy on the protective immunity in mice infected with malaria parasites, BALB/e mice infected intra-peritoneal with erythrocytes parasitized with Plasmodium yoelii 17XNL（non-lethai strain） were treated with different dose of Chloroquine or Artesunate Thirty days after infection during which the parasites inoculated were cleansed up in the spontaneously cured group, mice of different groups were re-infected with P. yoelli 17XNL, P. yoelii 17XL （lethal strain） or P. berghei ANKA （heterologous strain）. The level of parasitemia in mice during primary and ehaUenged infections was observed by mean of the Giemsa staining of thin blood smears, and the levels of IFN-7 in the supernarants of cultured splenic ceils and the specific antibody levels in serum were determined by ELISA. It was demonstrated that high levels of IFN-7 production were obvious during early stage of primary infection both in the spontaneously cured and various treated groups of mice, and then levels of the specific IgG antibodies against P. yoelii（non-lethal strain） increased remarkablly without significant difference among different groups. A complete resistance to the challenges with P. yoelli（non-lethal and lethal strains） could be demonstrated,but few of the re-infected mice showed low level of temporal parasitemia. However, the infected mice challenged with the heterologous strain of plasmodia were died completely. Taken together, it is evident that treatment of mice infected with palsmodia by Chloroquine or Artesunate shows no any effect on the acquirement of anti-malarial immunity and the maintenance of immuno-memory obtained and the specific IgG antibodies are the main immune effector molecules of host to resist re-infection.

关 键 词：药物治疗 保护性免疫 约氏疟原虫 

分 类 号：R328.3[医药卫生—人体解剖和组织胚胎学]