催化荧光法研究辅酶NADH的抗氧化性能  

Studies on the Antioxidative Properties of NADH with Spectrofluorimetry

作　　者：郑琦[1] 曹启花[2] 蔡汝秀[2] 

机构地区：[1]江汉大学化学与环境工程学院,湖北武汉430056 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2006年第2期133-136,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(20275027);湖北省自然科学基金资助项目(2004ABA182)

摘　　要：采用催化荧光法观察了3种不同类型的活性氧物质(H2O2,.OH,ONOO-)与辅酶NADH的相互作用.研究表明,NADH具有明显的清除活性氧的功能,且对不同类型活性氧的清除作用表现不同:对.OH的清除作用最为迅速,对H2O2,ONOO-的清除作用则较为缓慢.进一步的研究发现,生物体内存在的肌红蛋白因具有过氧化物酶的特性可使清除H2O2的反应大大加速.The interactions between NADH （nicotinamide adenine dinucleotide） and three kinds of ROS （reactive oxygen species）, i.e. H2O2, .OH and ONOO , were studied. It is found that NADH is capable of scavenging ROS, and the scavenging efficiency is varied toward different objects. Among the three kinds of ROS, OH is eliminated the most rapidly, and H2O2 and ONOO^- are eliminated relatively slower. Further studies show that myoglobin, which is an in vivo biomolecule, is able to accelerate the scavenging of H2O2 by NADH notably clue to its peroxidatic activity.

关 键 词：催化荧光法 NADH 抗氧化 

分 类 号：O657.32[理学—分析化学]