拟南芥有性生殖过程中花粉管生长及胚胎发育的形态特征  

Morphologic Character of Pollen Tube Growth and Embryo Development during Sexual Reproductive Process of Arabidopsis thaliana

作　　者：胡颖 赵洁[1] 

机构地区：[1]武汉大学生命科学学院/植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2006年第2期241-246,共6页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30370089;30521004);长江学者和创新团队发展计划资助项目(IRT0437)

摘　　要：以模式植物拟南芥(Columbia ecotype)为供试材料,利用脱色苯胺蓝染色的方法观察了拟南芥花粉管在雌蕊组织中的生长情况,获得了花粉管从萌发到进入花柱和子房不同时期的生长速率,结果表明,花粉管在不同时期的生长速率呈现出逐步加快的趋势.同时采用手工分离的方法分离了不同发育时期的胚胎,研究了胚胎发育随时间进程的形态结构特征,并与Landsberg生态型拟南芥的胚胎发育速率进行了比较,发现Columbia生态型的发育速率略慢.此外还测量了胚胎不同发育时期的角果长度,获得了胚胎发育时期与角果长度之间的对应关系,发现随着胚胎的发育,角果的生长速率呈现出逐渐递减的趋势.With the aid of aniline blue staining, pollen tube growth in pistil tissue was observed in Arabidopsis thaliana （Columbia ecotype）, and the growth speed of pollen tubes in style and ovary were calculated, the results showed that the pollen tube grew faster as time goes on. Furthermore, embryos at different developing stages were manually isolated to understand their changes of morphologic character along with the time course, then the developmental situation difference were compared between the Columbia ecotype and the Landsberg ecotype, the results showed that the developmental speed of the Columbia ecotype was slower than that of the Landsberg ecotype. Besides, the lengths of siliques at different developing stages were measured, and relation between silique length and time course were investigated, the results showed that the siliques grew slower as time goes on. The results could be valuable and reliable information for the reproductive developmental biology researches of the model plant Arabidopsis thaliana.

关 键 词：拟南芥 有性生殖 花粉管 胚胎发育 形态特征 

分 类 号：Q94[生物学—植物学]