马桑叶中总三萜酸的含量测定  

Determination of the Content of the Tritepenoidic Acid in Coriaria sinica Maxim

作　　者：张雁冰[1] 王克让[1] 刘宏民[1] 

机构地区：[1]郑州大学新药研究开发中心,河南郑州450052

出　　处：《时珍国医国药》2006年第4期529-530,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河南省科学自然科学基金资助项目(No.0311040900)

摘　　要：目的 测定马桑叶中总三萜酸的含量。方法 以熊果酸为对照品，以5％香草醛-冰乙酸和高氯酸为显色剂，采用紫外分光光度法在548nm波长出测定样品的吸光度。结果 总三萜酸在26．25—91．875μg范围内呈良好线性关系，其回归方程为），=0．0023x＋0．1404，r=0．9980。马桑叶中总三萜酸的含量约为2.0％。结论 紫外分光光度法适合马桑叶中总三萜酸的含量测定，该方法操作简便，结果稳定，重现性好。Objective To determine the content of the tritepenoidic acid in the leaves of Coriaria sinica Maxim. Methods Ursolic acid as the standard,5% vanillin- acetic acid and perchloric acid as the developer, used the ultraviolet spectrophotometric method to determine the content of the tritepenoidic acid in the leaves of Coriaria sinica Maxim. Results The calibration curve was linear （ r = 0. 998 0） in the range of 26.25 -91.875 μg, and the curve was y =0. 002 3x ＋0. 140 4,the content was about 2.0%. Conclusion The spectrophotometry is suitable for the determination of total tritepenoidic acid from the leaves of Coriaria sinica Maxim. This method operated convenientely, the result was stable, and has good repeatability.

关 键 词：紫外分光光度法 马桑 熊果酸 三萜酸 马桑叶 

分 类 号：R284.1[医药卫生—中药学]