荧光分光光度法测定氧氟沙星片剂的含量  

etermination of ofloxacin in tabletsby fluorospoectrophotometry

作　　者：刘新泳[1] 张君仁[1] 王德凤[1] 任健[1] 田心 

机构地区：[1]山东医科大学药物分析教研室

出　　处：《西北药学杂志》1996年第3期99-100,共2页Northwest Pharmaceutical Journal

摘　　要：用荧光分光光度法测定氧氟沙星片剂的含量。选用０．０５ｍｏｌ／Ｌ盐酸为溶剂，在Ｅｘ＝２９３ｎｍ、Ｅｍ＝５０７ｎｍ处测定荧光强度。在０．１０～０．３５ｕｇ／ｍｌ范围内，荧光强度与浓度呈线性关系，回归方程为Ｃ＝０．０７３６Ｆ—０．００６９（ｒ＝０．９９９８）。平均回收率为９９．５３％，ＲＳＤ为０．８９％（ｎ＝８）。本法灵敏度高，操作简便，结果准确。This article reported the assay of ofloxacin tablets by fluorospectrophotometry. Hy-drochloride acid solution was selected as solvent and Ex293nm and Em507nm were set. There was agood correlation between fluoresent value and concentration ranging from 0. 10μg/ml to 0. 35μg/ml.The regression equation was C= 0. 0736F- 0. 0069 (r= 0. 9998). The average recovery was 99.53 %, RSD= 0. 89 % (n= 8). This method is sample, sensitive and accurate.

关 键 词：氧氟沙星 荧光分光光度法 含量 

分 类 号：R927.2[医药卫生—药学]