cDNA微阵列检测苯中毒细胞增殖与周期调控基因差异表达  

cDNA microarray to identify cell proliferation and cell cycle genes associated with benzene poisoning

作　　者：张玲[1] 吕必华[2] 赵职卫[3] 夏颖[4] 张本延[1] 毕勇毅[3] 

机构地区：[1]武汉科技大学医学院预防医学系,430080 [2]武汉大学中南医院药学部 [3]武汉大学公共卫生学院劳动与环境卫生系 [4]湖北省疾病预防控制中心

出　　处：《公共卫生与预防医学》2006年第2期8-11,共4页Journal of Public Health and Preventive Medicine

基　　金：国家自然科学基金资助(30571556);湖北省自然科学基金(2001ABB161)

摘　　要：目的通过基因表达谱研究寻找苯中毒发病机制中细胞增殖与周期调控基因差异表达。方法应用含338条免疫相关基因的cDNA芯片检测7例苯中毒患者和7例正常人的外周血白细胞基因表达谱,观察其在基因表达谱上的差异。进行了基因表达谱聚类分析和比较;筛选有统计学意义的差异表达的基因。结果所有芯片中出现差异表达的基因共有44条,其中表达上调的基因17个,主要包括有IF IM 1、CDKN 1B、RBL 2、G SPT 1、FOXO 1A等;表达下调的基因17个,有CDC 37、M DM 4、EV I5、SKP 2等基因;表达不一致的基因6个。聚类分析揭示40条基因的基因表达谱特征存在关联。结论通过有统计学意义的差异表达的苯中毒相关基因,推测细胞增殖与细胞周期调控可能在苯中毒发病中起重要作用。Objective To detect cell proliferation and cell cycle genes associated with benzene poisoning by using gene expression profile analysis. Methods Peripheral white blood cell gene expression profile of 7 benzene poisoning patients ,including one aplastic anemia ,was determined by microarray. Seven chips from normal workers were served as control. Cluster analysis of gene expression profile was performed. Differentially expressed genes associated with benzene poisoning were determined. Results Among the 338 target genes, 40 genes differentially expressed were identified,including 17 up-regulated genes, mainly, IFIM1 ,CDKN1B,RBL2,GSPT1,FOXO1Aetc,and 19 down-regulated genes, mainly, CDC37,MDM4,EVI5,SKP2 etc, and other 6 genes expression showing difference. Cluster analysis showed that the expression profiles of 40 genes were associated with benzene poisoning. Conclusion Differentially pathways may play a important role in the pathogenesis of benzene poisoning.

关 键 词：苯中毒 cDNA微列阵 基因表达 

分 类 号：R854[医药卫生—航空、航天与航海医学] R730.21[医药卫生—临床医学]