微流控芯片分离效果光电检测方法研究  

Study on the Photoelectric Technique on Testing the Separation Effect by Microfluidic Chip

作　　者：刘岩[1] 左春柽[1] 陈洪利[1] 姜立标[2] 李月强[2] 

机构地区：[1]吉林大学机械科学与工程学院 [2]哈尔滨工业大学威海分校,山东威海264200

出　　处：《佳木斯大学学报（自然科学版）》2006年第2期174-176,共3页Journal of Jiamusi University：Natural Science Edition

基　　金：教育部博士基金;编号:20040183057

摘　　要：设计了一套用于微流控芯片分离效果检验的光学检测系统,系统使用生物落射荧光显微镜作为主要光路系统,用彩色CCD摄像头采集图像信息输入计算机,用线形灰度变换算法处理RGB24位彩色图像数据.用该系统对瓢虫翅膀、洋葱细胞、机油、聚氯乙稀颗粒和熔融玻璃管做了检测实验,分析了影响检测效果的因素.A microscope detecting system has been developed to detect the separating in electrophoresis chips. An epifluorescent microscope is selected as the main optical system, in which the analog images are collected by the color CCD camera and imported into computer after transformed into digital image by a video adapter card. Then the image process begins by the software developed by the authors. The 24bit RGB image data are conduced by means of hne - gray transforming method. The system has been apphed to detect ladybug＇s wing, onion lice, machine oil, PVC particles and frit micro - tube. Besides, the factors which affect the detecting result have been analyzed.

关 键 词：微流控芯片 光学检测 CCD 图像处理 

分 类 号：TP274.5[自动化与计算机技术—检测技术与自动化装置]