缬沙坦对兔主动脉PPAR-γ mRMA及其蛋白表达的影响  

作　　者：李西栋[1] 管思明[1] 王斌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《山东医药》2006年第13期12-13,共2页Shandong Medical Journal

基　　金：中央保健委员会保健专项资金科研项目(2002鄂C015)

摘　　要：目的研究缬沙坦对兔主动脉过氧化物酶体增殖物激活受体-γ(PPAR-γ)表达的影响,探讨其抗动脉硬化(AS)的可能机制。方法将24只新西兰兔随机分成正常对照组、高脂组、药物组,分别给予正常饮食、高脂饮食、高脂饮食加缬沙坦灌胃,共14周。采用逆转录聚合酶链反应及免疫组化法分别检测兔主动脉PPAR-γmRNA及其蛋白表达,并测量主动脉内膜厚度及内膜/中膜厚度比。结果药物组内膜厚度低于高脂组(P<0.05)。主动脉PPAR-γ表达药物组>高脂组>对照组,P均<0.05。药物组主动脉PPAR-γ蛋白表达高于高脂组(P<0.05),对照组未见明显表达。结论缬沙坦可能通过增加PPAR-γ表达而发挥抗AS作用。Objective： To study the effect of valsartan on the expression ot peroxisome proliferator-activated receptor-γ （PPARγ） in rabbit aorta, and investigate the possible anti-atherogenic mechanism of valsartan. Methods： Twenty four rabbits were randomly divided into 3 groups：the control group, cholesterol group and valsartan group. Three groups were fed with normal diet, high fat diet and high fat plus valsartan lavage for 14 weeks respectively. Then the mRNA of PPAR-γ was examined by semi-quantitative RT-PCR, and the protein of PPAR-γ was detected by imunohistochemistry. The aorta intimal thickness and intimal/medial thickness ratio were measured by image analysis system. Results： Intimal thickness in valsartan group was lower than that in the control group （P〈0.05）. The expression of PPAR-γ mRNA in cholesterol group was higher than that in the control group （P〈0. 05）, and it further increased after valsartan treatments （P〈0. 05）. The expression of PPAR-γ protein in valsartan group was higher than that in the cholesterol group （P〈0.05）, but was not definitely seen in the control group. Conclutions： Valsartan might have anti-atherosclerosis effect by upregulating the expression of PPAR-γ.

关 键 词：缬沙坦 过氧化物酶体类 动脉硬化 

分 类 号：R543.5[医药卫生—心血管疾病] R972[医药卫生—内科学]