RT-PCR法扩增人锰超氧化物歧化酶cDNA的克隆及其核苷酸序列测定  

Clonning and Sequencing or Human Manganese Superoxide Disnutase GenecDNA Amplified with RT-PCR from Human Liver Total RNA

作　　者：骆训懿[1] 周丽君[1] 陈晓穗[1] 王欲晓[1] 王晶翼[1] 谢邦铁[1] 何菱[1] 陈宇萍[1] 

机构地区：[1]海军总医院中心实验科

出　　处：《海军总医院学报》1996年第1期1-4,共4页Journal of Naval General Hospital of PLA

摘　　要：从人肝细胞提取总RNA,以此为模板进行逆转录-聚合酶链反应(RT-PCR),制备出人锰超氧化物歧化酶(hMnSOD)的cDNA片段.将此cDNA克隆入专为装载PCR产物而构建的质粒载体pGEM-T中.对重组质粒进行限制酶切分析和序列测定.结果表明,该克隆片段与国外报道的hMnSOD cDNA序列一致.表达蛋白的工作正在进行之中.The cDNA encoding human manganese superoxide dismutase was amplified from the human liver total RNA by RT-PCR method. The cDNA fragment was directly ligated imto pGEM-T and transformed into E. coli JM101. The cloned gene was analyzed by restriction en-zymes EcoR I, Sal I, and BamH I. The sequence of the cloned gene was determined.

关 键 词：分子克隆 序列测定 hMnSOD CDNA 

分 类 号：R977.3[医药卫生—药品] R965.2[医药卫生—药学]