头孢他定与FALL-39用于脓毒血症的疗效  

The treatment effects of FALL-39 peptide and cefatazigine on sepsis

作　　者：冯新富[1] 石承先[1] 陈晓理[2] 李俊华[1] 王伯瑶[3] 

机构地区：[1]贵州省人民医院普通外科,贵阳550001 [2]四川大学华西医院普通外科 [3]四川大学华西基础医学院感染免疫室

出　　处：《江苏医药》2006年第5期462-464,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(39970932)

摘　　要：目的观察FALL-39和头孢他定（CFZ）治疗脓毒血症的疗效。方法从pGEX-1λT-FALL-39转染的E.coli JM109中提纯FALL-39，检测FALL-39对绿脓杆菌（PAG）的杀菌活性。采用更生霉素（AMD）增强BALB／c小鼠对PAG感染的敏感性，建立脓毒血症模型，观察FALL-39和CFZ治疗脓毒血症的疗效。结果FALL-39分子量约5kDa，纯化量约600-650μg，对PAG的最低抑菌浓度（MIC）、最低有效浓度（MEC）、最低杀菌浓度（MBC）分别为25、100、50μg／ml。盐水组、脓毒血症组、CFZ治疗组、FALL-39治疗组和CFZ＋FALL-39治疗组小鼠死亡率分别为0％、75％、62．5％、37．5％和12．5％。结论FALL-39治疗脓毒血症比CFZ更为有效，两者联用效果则更佳。Objective To investigate the effects of FALL-39 peptide and cefatazigine on treating sepsis. Methods Purified FALL-39 was extracted from Escherichia coli JM109 transfected by the pGEX-1λT-FALL-39 recombinant vector. The antibacterial activity of FALL-39 was verfied by measuring the MIC, MEC, MBC for P. aeruginosa. The infectional sensitivity of BALB/c mice for P. aeruginosa was induced by employing actinomycin D and the actinomycin-sensitized BALB/C murine sepsis model was constructed. The treatment effects of FALL-39 peptide and cefatazigine on BALB/C murine sepsis model were investigated. Results The molecular magnitude of FALL-39 was identified approximate 5kDa. About 600- 650μg of highly purified FALL-39 was obtained. The antibacterial activity of FALL-39 was determined,and its minimal effective concentration（MEC）,minimal inhibitory concentration（MIC）, and minimal bactericidal concentration（MBC） were 25,100, and 50μg/ml, respectively. Death rates of saline, sepsis, CFZ, FALL-39 recombinant peptide and CFZ＋FALL-39 subgroups were 0%,75%,62. 5%,37.5%,and 12.5%,respectively. Conclusion FALL-39 recombinant peptide is more effective than ceftazigine in treating sepsis and the efficacy of combining ceftazigine with FALL-39 recombinant peptide in treating sepsis is more than FALL-39 or ceftazigine alone.

关 键 词：头孢他定 FALL-39 脓毒血症 

分 类 号：R978.16[医药卫生—药品] R631[医药卫生—药学]