骨桥蛋白介导类风湿关节炎发病机制的初步研究  

The pathogenesis of osteopontin in rheumatoid arthritis

作　　者：许荣 钟丽民 袁斌 周丽萍 何东林 候娜莉[2] 李宁丽[2] 王利[2] 陈广洁[2] 余奇文[2] 张冬青[2] 

机构地区：[1]上海光华中西医结合医院类风湿关节炎实验室,200052 [2]上海第二医科大学上海市免疫学研究所

出　　处：《中华风湿病学杂志》2006年第5期261-264,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目(30471593);上海市重大课题资助项目(03DJ14009);上海市教委重点项目(03BZ02);上海市教委第4期重点学科资助项目(ZDXK2001);上海第二医科大学基础医学院合作研究基金资助项目(2004JY01)

摘　　要：目的研究骨桥蛋白(OPN)介导类风湿关节炎(RA)发病和促进炎症因子产生的作用。方法采用实时定量聚合酶链反应(PCR)的方法检测OPN在RA患者外周血、关节液和关节滑膜组织和在T淋巴细胞亚群中的表达;从蛋白水平分析RA患者关节液中促炎症细胞因子和抗炎症细胞因子的分泌格局,分析OPN与细胞因子的相关性。结果RA患者关节滑液和关节滑膜组织的OPN表达远远高于外周血单个核细胞的OPN基因表达,CD4+T细胞OPN基因表达均高于CD8+T细胞;RA患者关节液中OPN的表达显著高于血清,白细胞介素(IL)-10,干扰素(IFN)-γ和肿瘤坏死因子(TNF)-α水平比自身血清及对照组血清增高,IL-18和IL-12水平比正常对照组明显升高。结论OPN可能通过调节病灶部位的细胞因子的分泌来调控RA病理过程中的炎症反应。Objective To study regulatory mechanism of osteopontin （OPN） in rheumatoid arthritis （RA）. Methods The expression of OPN in peripheral blood mononuclear cells （PBMC）, synovial fluid cells （SFMC） and synovium tissue （ST） and T cell subsets from RA patients were detected by real time PCR. The concentration and relative rate of inflammatory factors in the synovial fluid from RA patients were analyzed by ELISA. Results The mRNA expression of OPN in synovial fluid and tissue was higher than that of PBMC in the same RA patient. The OPN expression was found mainly on CD4^＋T. The OPN concentration was higher in the synovial fluid than that of in the same patient＇s serum. Meanwhile, the concentration of IL-10, IFN-γ and TNF-α was higher than that of in the serum from same patient. Also, the concentration of IL-18 and IL-12 were higher than that of normal individual serum. Conclusion OPN may control secretion of inflammatory factors of synovium tissue and synovial fluid and induce the inflammatory response.

关 键 词：关节炎 类风湿 骨桥蛋白 细胞因子 发病机制 

分 类 号：R593.22[医药卫生—内科学] R543.5[医药卫生—临床医学]