D-核糖生产菌的原生质体诱变及其发酵培养  

Protoplasts Mutagenesis and Fermentation of D-Ribose Producing Strains

作　　者：常景玲[1] 张志宏[1] 刘学良[1] 

机构地区：[1]河南科技学院生物工程系,河南新乡453003

出　　处：《生物技术通讯》2006年第2期195-197,共3页Letters in Biotechnology

基　　金：河南省科技厅科技攻关项目(0424240031)

摘　　要：目的:筛选出莽草酸、转酮醇酶双重营养缺陷型的D-核糖生产菌枯草芽胞杆菌,以提高D-核糖的产量。方法:采用化学诱变剂乙基磺酸甲烷(EMS)对野生型D-核糖生产菌的原生质体进行诱变,并从D-核糖合成途径对发酵培养基进行优化设计。结果:摇瓶发酵D-核糖平均产量为52.2g/L;获得的B.sems-10菌株具有良好的遗传稳定性,D-核糖产量高达67.5g/L。结论:通过对D-核糖生产菌原生质体的EMS诱变,筛选出了高产、遗传性状稳定的营养缺陷型B.sems-10菌株,为进一步提高产量奠定了基础。Objective： To obtain the strains of oxalic acid and ketone alcohol enzyme double nutrition defected and improving D-ribose output of Bacillus subtilis. Method： EMS was used as mutagen in treating protoplasts of original strains and the culture medium was optimized. Results： The average output was 52.2 g/L in reeling bottle condition and the high output was up to 67.5 g/L under the same condition, the strain B.sems-10 has good stability of heredity. Conclusion： It is a effective way to obtain hige D-ribose output producer compared with the original strain by using EMS as mutagen in treating protoplasts of original strain from Bacillus subtilis.

关 键 词：D-核糖 枯草芽胞杆菌 原生质体 诱变 发酵 

分 类 号：Q813.5[生物学—生物工程]