FK506在异体神经移植过程中对雪旺细胞和巨噬细胞的影响  

Effect of immunosuppressive drugs FK506 on allogenic nerve transplantation after peripheral nerve injury

作　　者：杨俊 张振伟[2] 朴英杰[3] 钟世镇[3] 秦建强[3] 

机构地区：[1]解放军第一八八医院广州军区创伤骨科中心,广东潮州521000 [2]深圳市沙井人民医院手外科治疗康复中心 [3]南方医科大学临床解剖学研究所

出　　处：《中华创伤骨科杂志》2006年第5期448-452,共5页Chinese Journal of Orthopaedic Trauma

基　　金：广东省科技计划重大专项(2003A3020101);广东省重点实验室项目(2001B60107)

摘　　要：目的从形态学角度研究FK506在异体神经移植后神经修复过程中对雪旺细胞增殖和巨噬细胞凋亡的影响。方法体外培养纯化雪旺细胞,流式细胞仪检测FK506促雪旺细胞增殖和促巨噬细胞凋亡作用,ELISA法检测FK506促雪旺细胞分泌神经生长因子(NGF)及促巨噬细胞分泌白细胞介素-1(IL-1)的含量,荧光显微镜和透射电镜对巨噬细胞的凋亡进行形态学观察与鉴定。结果流式细胞仪和ELISA检测结果显示FK506可促进雪旺细胞增殖并分泌NGF,促进巨噬细胞凋亡并分泌IL-1,荧光显微镜和透射电镜下观察到巨噬细胞凋亡前期和凋亡小体的出现。结论在形态学角度FK506可以在早期促进雪旺细胞增殖和分泌NGF,并促进巨噬细胞凋亡和IL-1的分泌,进而促进外周神经再生。Objective To investigate and confirm morphologically the effect of FK506 on promoting proliferation of Schwann cells and apoptosis of macrophages during the repair progress after an allogenic nerve transplantation for a peripheral nerve injury. Methods Schwann cells were cultured and purified in vitro. Flow cytometry was used to determine FK506＇s effect on promoting proliferation of Schwann cells and apoptosis of macrophages. ELISA （enzyme linked immunosorbent assay） was performed to determine how FKS06 influenced nerve growth factor （NGF） expression level of Schwann cells, and affected interleukin-1 （IL-1 ） expression level of macrophages. Morphological evaluation was done for the apoptosis of macrophages by transmission electron microscope and fluorescence microscope. Results FK506 was observed to promote proliferation and NGF expression of Schwann cells, and to promote apoptosis and IL-1 expression of macrophages. Transmission electron microscope and fluorescence microscope observations revealed apoptosis prophase and apoptosis body of macrophages. Conclusions Since FK506 can promote proliferation of Schwann cells and apoptosis of maerophages at an early stage, it facilitates the repair progress of allogenic nerve transplantation and reduces or suppresses immunological rejection caused by activated macrophages after peripheral nerve injury.

关 键 词：免疫抑制剂FK506 雪旺细胞 巨噬细胞 外周神经 增殖 凋亡 白介素-1 

分 类 号：R651.3[医药卫生—外科学]