IL-13中和抗体对哮喘小鼠恢复期气道炎症及Th1/Th2功能的影响  

Effects of IL-13 neutralization antibody on airway inflammation and Th1/Th2 cell function during convalescent period of asthma in mice

作　　者：田代印[1] 符州[1] 王莉佳[1] 何云锋[1] 王成秀[1] 周娟[1] 罗晓菊[1] 

机构地区：[1]重庆医科大学附属儿童医院呼吸科,重庆400014

出　　处：《免疫学杂志》2006年第3期302-304,共3页Immunological Journal

基　　金：国家自然科学基金资助项目(30271380)

摘　　要：目的探讨白细胞介素13(IL-13)中和抗体对哮喘小鼠恢复期气道炎症及Th1/Th2功能的影响。方法Balb/c小鼠24只,随机分为3组,即对照组、模型组、治疗组。模型组和治疗组用鸡卵清蛋白(OVA)致敏和激发,建立哮喘小鼠模型,对照组用生理盐水代替OVA。治疗组自最后一次激发后24 h起,每48 h注射IL-13中和抗体1次,连续14 d,对照组和模型组则用生理盐水替代。结果治疗组肺组织病理切片较模型组相比,炎症细胞明显减少,肺气肿程度明显减轻。血清及支气管肺泡灌洗液(BALF)中,治疗组较模型组IL-4、OVA特异性IgE明显下降,IFN-γ明显增加,基本接近正常对照组。结论IL-13中和抗体对哮喘小鼠恢复期有明显的治疗作用,除能明显减轻气道炎症和肺气肿外,还能纠正哮喘小鼠体内存在的Th1/Th2细胞因子失调。Objective To investigate the effects of IL-13 neutralization antibody on the airway inflammation and Th1/Th2 cell function after acute attack of asthmatic mice. Methods A total of 24 Balb/c mice were randomly divided into 3 groups： the control group, asthmatic group, and treatment group. Mice in the latter 2 groups were sensitized and challenged with ovalbumin （OVA） to establish asthmatic models, while mice in control group were treated with saline as the substitution of OVA. After the last OVA challenge, mice in treatment group were in jeered with the IL-13 neutralization antibody per 48 h for 14 days, while mice in control group and asthmatic group received saline. Results The inflammatory cell recruitment and pulmonary emphysema were greatly attenuated in the treatment group. In either blood or bronchoalveolar iavage fluid （BALF）, the IFN-γ level was significantly elevated but the IL-4 and OVA specific IgE levels were much lower in the treatment group compared with the asthmatic group. There were not apparent differences between the treatment group and the control group. Conclusion This study demonstrates that IL-13 neutralization antibody works effectively after acute attack of asthmatic mice, not only attenuating the airway inflammation and pulmonary emphysema, but also regulating the disorder of Th1/Th2 cytokines in asthmatic mice.

关 键 词：IL-13 抗体 哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]