三叶半夏组培苗生长情况调查  

Investigation on the Growth of the in vitro Regenerated Plantlets of Pinellia ternata

作　　者：蔡鹏[1] 彭正松[1] 罗成科[2] 魏淑红[1] 杨军[1] 

机构地区：[1]西华师范大学生命科学学院,环境科学与生物多样性保护省重点实验室,四川南充637002 [2]宁夏大学新技术应用研究开发中心,宁夏银川750021

出　　处：《现代中药研究与实践》2006年第2期16-19,共4页Research and Practice on Chinese Medicines

基　　金：四川省杰出青年基金后续项目(05ZQ026-041);四川省重点学科建设项目(SZD0420)

摘　　要：目的为半夏快速繁殖技术体系的建立提供理论依据。方法通过一步成苗法获得半夏组培苗后进行移栽实验,并进行形态学观察和产量统计。结果半夏组培苗三种不同叶形的叶柄上均可萌生珠芽,块茎越大的植株大多出现三出复叶的时间较早,其产生的珠芽数也越多,且在移栽的当年就可长出佛焰苞;在一个生长期之后,母块茎的收获重量可达到栽种量的31.7倍;两批半夏组培苗的无性繁殖系数分别达到了12.8个/株和10.1个/株。结论半夏组培苗具有生长速度快,繁殖系数高的特点。Objective To provide more scientific foundation for establishment of rapid propagation system of Pinellia ternata. Methods The in vitro regenerated P. ternata plantlets which were cultured by one-step culture method were transplanted in the flowerpots. Their biological characters were observed, and their yield had made statistical. Results It was found that bulbils could sprout from the petioles of all the three kinds of leaves. Trifoliate leaf outgrew from the bigger mother tubers earlier, and more bulbils spouted from these tubers. And these plants could grow spathes in the primary growing period. Fresh weight of harvested mother tubers is 31.7 times of the sowed weight after one growing period, propagation coefficient of two group was high up to 12.8 and 10.7 per plant,respectively. Conclusion The in vitro regenerated plantlet of P. ternata have the characters of rapid growth and high propagation coefficient.

关 键 词：三叶半夏 组培苗 珠芽 繁殖系数 

分 类 号：R169.42[医药卫生—公共卫生与预防医学] R282.21