一个水稻NADPH氧化还原酶相似基因的克隆及表达分析  

Cloning and Expression Analysis of a NADPH Oxidoreductase-Like Gene from Rice

作　　者：陈静[1] 姜华[1] 万佳[1] 高晓玲[1] 王平荣[1] 席江[1] 徐正君[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130

出　　处：《中国水稻科学》2006年第3期238-242,共5页Chinese Journal of Rice Science

基　　金：国际合作资助项目(05GH0001);教育部长江学者和创新团队发展计划(IRT0453);四川省青年科技基金资助项目

摘　　要：采用荧光差显(fluorescent differential display,FDD)技术,比较分析了干旱处理与未处理水稻叶片及根中的mRNA表达,并利用H.A.Yellow-PAGE(含0.1%H.A.Yellow)再分离与大矩阵(macroarray)筛选相结合的方法,发现1个与拟南芥NADPH氧化还原酶高度相似(96%)的差异片段。该基因的cDNA全长为1423bp,编码一个具有345个氨基酸残基的多肽。在正常情况下该基因表达量很低,而在干旱处理中高表达。讨论了干旱胁迫下NADPH氧化还原酶基因的可能功能。By using a fluorescent differential display method, the mRNA expression difference in rice leaves and roots under drought stress and normal conditions was compared. One positive fragment was isolated by combining the H. A. Yellow- PAGE （containing 0. 1% H. A. Yellow） separation with macroarray screening methods. The gene has 96% identity to the Arabidopsis thaliana NADPH oxidoreductase gene. The cDNA was 1423 bp in size, including a complete open reading frame of 1048 bp encoding a protein with 345 amino acid residues. The gene expression level was higher under drought stress than that under normal conditions. The possible role of NADPH oxidoreductase-like gene under drought stress was discussed.

关 键 词：水稻 干旱胁迫 NADPH氧化还原酶相似基因 克隆 基因表达 

分 类 号：S511[农业科学—作物学]