空气中CO_2浓度升高条件下水稻抽穗期的QTL定位  

QTL Analysis for Heading Date of Rice under Free Air CO_2 Enrichment Conditions

作　　者：范桂枝[1] 李军营[1] 王春明[2] 谢辉[1] 徐长亮[1] 朱建国[3] 万建民[2] 蔡庆生[1] 

机构地区：[1]南京农业大学生命科学学院 [2]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [3]中国科学院南京土壤研究所,江苏南京210008

出　　处：《中国水稻科学》2006年第3期259-264,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30270800;40231003)

摘　　要：以65个水稻染色体片段置换系(chromosome segment substitutionlines,CSSLs)为材料,对比分析了正常大气CO2浓度(对照)和开放式空气CO2浓度升高(free air CO2enrichment,FACE,大气CO2浓度增加200μmol/mol)下水稻抽穗期的变化,并定位了相关QTL(quantitative trait loci)。供试株系的抽穗期对CO2浓度升高表现为提早、延迟和不变等3种响应,抽穗期两极变化最大的株系为AI46(提前11d)和AI63(延迟6d)。两种条件下,共检测到9个控制抽穗期的QTL,分布在第3、4、6、7、8、10和11染色体上。其中位于第6和第8染色体上的qHD6-4和qHD8-4在两种CO2浓度下都检测到,但在FACE下的贡献率均显著增大;而qHD3A-3和qHD11A-7只在正常条件下检测到,qHD4F-4、qHD10F-4和qHD11F-4则只在FACE下检测到。暗示控制水稻抽穗期的基因表达易受环境CO浓度的影响。A mapping population consisting of 65 chromosome segment substitution lines（CSSLs） was used to detect quantitative trait loci （QTL） for heading date of rice （Oryza sativa L . ） under the ambient atmosphere CO2 concentration （Ambient） and free air CO2 enrichment （FACE, ambient ＋ 200μmol/mol） conditions. Both positive and negative responses to elevated CO2 at heading date of CSSLs were found. Among them, two CSSLs, A146 （advanced 11 days） and A163 （delayed 6 days）, were the most sensitive to elevated CO2 in different directions. A total of 9 QTLs for heading date were detected on chromosomes 3, 4, 6, 7, 8, 10 and 11 under the FACE and Ambient. Of them, qHD6^-4 and qHD8^-4 were simultaneously detected under both the FACE and Ambient, but the percentage of variation explained under the FACE was much higher than that under the Ambient, in addition, two QTLs, qHD3^A-3 and qHD11^A-7 were only detected under Ambient, and three QTLs, qHD4^F-4 , qHD10^F-4 and qHD11^ F-4 only under FACE, suggesting that the genes affecting heading date were sensitive to atmosohere CO2 concentration.

关 键 词：CO2浓度 水稻 染色体片段置换系 抽穗期 数量性状座位 

分 类 号：S511[农业科学—作物学]