转反义Wx基因水稻颖果的发育及物质积累  

Caryopsis Development and Substance Accumulation of Transgenic Rice with Antisense Wx Gene

作　　者：陈刚[1] 王忠[1] 刘巧泉[1] 熊飞[1] 顾蕴洁[1] 顾国俊[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《中国水稻科学》2006年第3期277-282,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30471045;3030026);国家863计划资助项目(2004AA212092);扬州大学高层次人才科研启动基金资助项目

摘　　要：以转反义Wx基因粳稻和籼稻品系为材料,研究其颖果的发育和物质积累。结果表明,直链淀粉含量降低后的转基因水稻品系籽粒的粒重会有所下降,而且直链淀粉含量下降越多,粒重的下降幅度也越大。单个颖果胚乳细胞的数目也有不同程度的下降,但在籽粒发育早期(花后6d前),转基因水稻的胚乳细胞增殖速率明显高于其亲本。直链淀粉降低后的转基因品系籽粒可溶性糖含量在发育初期(花后9d前)低于其同时期的亲本,而花后9d后则明显高于其亲本。转基因水稻籽粒中的总淀粉含量有不同程度的下降,而支链淀粉的含量却相对增加,从而改变了籽粒中淀粉的组成,但对籽粒中蛋白质的积累没有显著影响。The development and substance accumulation of rice caryopsis were studied by using the transgenic japonica and indica rice lines with antisense Wx gene. The weights of caryopses in the transgenic rice were lower than those of the non transgenic ones, and the reduction of weight was significantly correlated with the degree of reduction in amylose content. In the caryopsis of the transgenic rice, the number of endosperm cells was less, but the proliferation speed was obviously higher compared with non-transgenic ones during the first six days after flowering （DAF）. During the first nine DAF, the content of soluble sugar in the caryopsis of the transgenic rice was less than that in non-transgenic ones, but it was reversed after 9 DAF. With the decrease of amylose content in the caryopsis of the transgenic rice, the total starch content also declined while the amylopectin content increased accordingly, so the composition of starch in caryopsis was changed, but it did not affect the accumulation of protein in the caryopsis of the transgenic rice.

关 键 词：水稻 转基因 颖果 发育 物质积累 蜡质基因 

分 类 号：S511[农业科学—作物学]