水稻上两类人体条件致病细菌的鉴别与检测  

Identification and Detection of Two Types of Opportunistic Human Bacterial Pathogens from Rice

作　　者：郝晓娟[1] 谢关林[1] 吕意琳[1] 罗远婵[1] 张立新[1] 罗金燕[1] 赵丽涵[1] 刘少颖[1] 

机构地区：[1]浙江大学水稻生物学国家重点实验室,浙江杭州310029

出　　处：《中国水稻科学》2006年第3期324-328,共5页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30370951);国家863计划资助项目(2002AA241261)

摘　　要：洋葱伯克氏菌(Burkholderiacepacia,Bc)和铜绿假单胞菌(Pseudomonas aeruginosa,Pa)既是农业上的生防菌又是医院的人体条件致病菌,了解它们的特征及在水稻上的分布情况是有效管理和利用这类原核生物的关键。在过去的9年中,利用常规细菌学、致病性测定、Biolog和脂肪酸分析(FAME)对采自平原、丘陵和山区稻区的631份稻谷样本和117份稻株样本进行了两类人体条件致病细菌的鉴定和检测研究。Bc菌和Pa菌可通过培养性状和常规细菌学方法作初步鉴别,Biolog和FAME能把它们鉴定到种,但Bc菌的基因型难以区分。Bc菌和Pa菌主要存在于水稻的根部;Bc菌和Pa菌在浙江的平原稻区分布最广,分别达6.1%和16.1%;在山区稻区最低,分别为1.0%和7.8%。Burkholderia cepacia （Bc） and Pseudomonas aeruginosa （Pa） are both biocontrol agents in agriculture and opportunistic human pathogens in hospitals. It＇s the key point to understand their characteristics and distribution in rice so as to effectively manage and utilize them. In the past nine years, the two opportunistic human pathogens were identified and detec ted from 631 rice seed samples and 117 rice plant samples from plain, highland and mountain of rice growing areas in China. Bc and Pa could be primarily differentiated by common bacteriological characteristics and pathogenicity tests and identified into species level by Biolog and FAME. However, the genotypes of Bc still could not be distinguished. Bc and Pa mainly existed in rice roots. The distribution of Bc and Pa in the rice growing areas was the highest in the plain, being 6.1% and 16. 1% , and the lowest in the mountain, being 1.0% and 7.8%, respectively.

关 键 词：洋葱伯克氏菌 铜绿假单胞菌 人体条件致病细菌 生物安全 

分 类 号：S476[农业科学—农业昆虫与害虫防治] R378[农业科学—植物保护]