白花前胡提取液含药血清对ET-1诱发体外培养心肌细胞肥大及凋亡的影响  

Effect of serum containing Pd-E on cultured hypertrophy cardiomyocytes and apoptosis stimulated by ET-1

作　　者：涂乾[1] 涂欣[2] 田齐武[1] 全国芳[1] 

机构地区：[1]江汉大学医学与生命科学学院,湖北武汉430056 [2]华中科技大学,湖北武汉430076

出　　处：《山东中医杂志》2006年第5期342-344,共3页Shandong Journal of Traditional Chinese Medicine

基　　金：武汉市科技攻关计划项目(编号:20025007170)

摘　　要：目的:探讨白花前胡提取液含药血清抗内皮素-1(ET-1)促发心肌细胞肥大效应及其对肥大心肌凋亡影响。方法:采用乳鼠心肌细胞体外培养纯化,比较白花前胡提取液含药血清对内皮素诱导体外培养心肌细胞活性、心肌细胞表面积,以及对肥大心肌细胞凋亡与凋亡相关基因Bcl-2/Bax表达的影响。结果:白花前胡提取液含药血清能够有效缩小ET-1促发的体外培养肥大心肌细胞表面积,降低肥大心肌细胞搏动频率,抑制肥大心肌细胞凋亡以及改善Bcl-2/Bax蛋白表达的失衡状态。结论:白花前胡提取液含药血清能有效降低心肌细胞对ET-1刺激的反应性,遏制心肌肥厚、心室重塑以及细胞凋亡,有调节因ET-1刺激引起的心肌细胞凋亡相关基因表达改变的作用。Objective. To study the effect of serum containing Pd-E on cultured hypertrophy cardiomyocytes and apoptosis stimulated by ET-1. Methods： Using the cultured neonatal rats ventricular myocyte, the cardiomyocyte size, pulsating frequency,content of DNA in cardiomyocyte and Bcl-2,Bax protein expression were examined. Result： Serum containing Pd-E has the effects of decreasing cardiomyocyte cell size, pulsating frequency, inhabitation the apoptosis and regulating the expression of Bcl-2, Bax protein which increase after ET-1 stimulation. Conclusion： Pd E might inhabitate the cardiac remodeling and modulating the expression of genes ralated to apoptosis. The striking ability of PdE to inhibit the effects of ET-1 on cadiomytocyte hypertrophy suggests that it could be an effective therapeutic drug in treating the contractile defects associated with cardiac hypertrophy and failure.

关 键 词：心力衰竭 白花前胡提取液 心肌重塑 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]