Influence of Low Cetyltrimethylammonium Bromide Concentration on the Interactions and Properties of Hemoglobin with Acyclovir  

作　　者：刘天睛 郭荣 

机构地区：[1]School of Chemistry and Chemical Engineering, Nanjing University, Jiangsu 210093, China [2]School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 250002, China

出　　处：《Chinese Journal of Chemistry》2006年第5期620-626,共7页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20573091, 20233010）.

摘　　要：The effects of cetyltrimethylammonium bromide （CTAB） on the properties of hemoglobin （Hb） at low CTAB concentration were studied in Hb/acyclovir/CTAB system by the methods of UV-Vis spectrum, fluorescence, zeta potential, conductivity and negative-staining transmission electron microscope （TEM）. With the increase of CTAB concentration, the UV peak intensity at 276 nm, the intrinsic fluorescence, the zeta potential of Hb and the system conductivity were all enhanced. Hb was easily oxidized to oxyHb and hemichrome. In Hb/acyclovir/CTAB system, CTAB made the UV-Vis spectrum, fluorescence, conductivity and conformation of Hb tend to be returned to those of the original Hb but the zeta potential not to do so. The UV absorption peak of Hb-acyclovir complex disappeared and the tight structure of Hb aroused by acyclovir was refolded. When CTAB concentration was higher than 5 × 10 ^5 mol/L, the two absorption peaks at 536 and 576 nm appeared again, and the Hb structure became looser again.The effects of cetyltrimethylammonium bromide （CTAB） on the properties of hemoglobin （Hb） at low CTAB concentration were studied in Hb/acyclovir/CTAB system by the methods of UV-Vis spectrum, fluorescence, zeta potential, conductivity and negative-staining transmission electron microscope （TEM）. With the increase of CTAB concentration, the UV peak intensity at 276 nm, the intrinsic fluorescence, the zeta potential of Hb and the system conductivity were all enhanced. Hb was easily oxidized to oxyHb and hemichrome. In Hb/acyclovir/CTAB system, CTAB made the UV-Vis spectrum, fluorescence, conductivity and conformation of Hb tend to be returned to those of the original Hb but the zeta potential not to do so. The UV absorption peak of Hb-acyclovir complex disappeared and the tight structure of Hb aroused by acyclovir was refolded. When CTAB concentration was higher than 5 × 10 ^5 mol/L, the two absorption peaks at 536 and 576 nm appeared again, and the Hb structure became looser again.

关 键 词：HEMOGLOBIN cetyltrimethylammonium bromide ACYCLOVIR PROPERTY INTERACTION 

分 类 号：R446.113[医药卫生—诊断学]