一种快速提取甘薯基因组DNA方案的建立  

A Method for Rapid Isolation of Genomic DNA in Sweetpotato on Small Scale

作　　者：吴秋云[1] 罗文彬[1] 邱永祥[1] 蔡南通[1] 

机构地区：[1]福建省农业科学院作物研究所,福建福州350013

出　　处：《山西农业大学学报（自然科学版）》2006年第2期119-120,124,共3页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：福建省科技重大专项(2004NZ01)

摘　　要：建立了一种小量快速提取甘薯基因组DNA的方法。用该法以甘薯幼叶为实验材料,用小量快速法提取甘薯基因组DNA。提取的DNA经酶切、琼脂糖凝胶电泳检测,结果表明DNA质量较好,所提取的DNA可用于RAPD、AFLP等技术。此方法具有提取速度快、利于规模化提取、DNA质量好和经济实用等优点,为甘薯及其它相关植物基因组DNA的提取提供了一种快速、简便的途径。A new method for rapid isolation of genomic DNA on small scale in sweetpotato was established. Genomic DNA from young leaves of sweetpotato was extracted by a new small scale and rapid isolating method. The extracted DNA by the new method was in a good quality, and could be used in RAPD, SSR and AFLP. The method displayed many fine characteristics such as high efficiency, good quality, practicality and so on. It is a good way for extracting genomic DNA from sweetpotato and other plants.

关 键 词：甘薯 基因组DNA提取 快速法 

分 类 号：Q781[生物学—分子生物学]