油茶种质资源遗传多样性分析  

Analysis of Genetic Diversity in Camellia oleifera Germplasms

作　　者：黄永芳[1] 陈锡沐[1] 庄雪影[1] 雷治国[1] 陈永忠[2] 彭邵峰[2] 

机构地区：[1]华南农业大学林学院,广州510642 [2]湖南省林业科学院,长沙410004

出　　处：《林业科学》2006年第4期38-43,共6页Scientia Silvae Sinicae

基　　金：广东省科技攻关项目(2002C2010603);广东省林业科技攻关项目(2002-13)。

摘　　要：利用RAPD标记技术分析90份油茶种质的遗传多样性,探讨无性系间的亲缘关系,以及抗病性、果油率和产油量3个数量性状与谱带的相关性。结果表明:RAPD扩增得到593条谱带,多态性谱带564条,多态比率达95·11%,特异性及特异性缺失谱带32条,占5·32%,无性系间存在丰富的遗传多样性;广西软枝油茶和广东阳春油茶均聚在同一亚类,从分子水平说明了阳春油茶和软枝油茶的亲缘关系比较近,可能是同一个农家品种;与抗病性、果油率和产油量3个数量性状显著相关谱带分别为13·1%、25·2%和34·5%,这些与抗病性、果油率和产油量数量性状相关谱带的确定,可为选育丰产、抗病、优质的油茶品种提供参考。The genetic diversity of 90 Camellia oleifera clones was investigated and the relationship between these clones was analyzed using RAPD technique. Correlations between three quantitative traits （disease resistance, the rate of oil and oil yield） and molecular markers were also analyzed. The results are as follows： The total number of amplified fragments is 593, among which there are 564 polymorphic bands, the percentage of polymorphic bands is 95.11%. There are 32 distinctive bands （including missing distinctive bands）, accounting for 5.32% in total bands. Abundant genetic diversity exists among C. oleifera clones. ‘Yangchun＇ and ‘Ruanzhi＇ of C. oleifera aggregated in the same subgroup, indicating that they have close relationship, and could be the same agronomic cultivar at molecular level. Bands related to disease resistance, the rate of oil and oil yield account for 13.1%, 25.2% and 34.5 % respectively, and could be used as reference in selecting resistant C. oleifera cuhivars with both high quality and high yield.

关 键 词：油茶 RAPD 遗传多样性 种质资源 

分 类 号：S718.46[农业科学—林学]