花生种子白藜露醇的诱导与抗黄曲霉侵染关系的研究  

Differential induction of resveratrol in susceptible and resistant peanut seeds infected by Aspergillus flavus

作　　者：梁炫强[1] 周桂元[1] 邹世春[2] 

机构地区：[1]广东省农业科学院作物研究所,广东广州510640 [2]中山大学化工学院,广东广州510275

出　　处：《中国油料作物学报》2006年第1期59-62,共4页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金项目(30571179);广东省科技计划项目(2003B21203;2004B50201005);广东省农业科学院攻关项目(04-攻关-02A)

摘　　要：通过比较抗—感花生品种受黄曲霉菌侵染后,种子诱导白藜露醇的差异,探讨白藜露醇诱导与花生种子抗黄曲霉侵染关系。结果表明:受黄曲霉侵染后,花生种子白藜露醇诱导的速度与抗性有关。抗性品种在受侵染后3d种子内的白藜露醇大量累积并达到峰值,含量提高30倍;而感病品种则在受侵染后4d才达到此峰值。放线菌素酮和放线菌素D处理后接种试验表明,种子本身含有合成白藜露醇的mRNA,当受外源黄曲霉菌侵染时能激活与合成白藜露醇相关酶的活性,合成大量白藜露醇。Resveratrol is one of phytoalexin compounds in peanut seed, which can be elicited by slicing and inoculation with Aspergillus flavus. GC - MS analysis of resveratrol from resistant/susceptible peanut seeds after inoculation with Aspergillusflavus showed that the resistance to Aspergillus flavus invasion was related to their capacity to synthesize resveratrol. The accumulation of resveratrol in resistant genotypes reached the maximum at 3d after inoculation, whereas in susceptible genotypes it roached the same level at 4d. Cyclohexnomycin at a concentration of 100μg/mL caused a 93% decrease in total resveratrol production, while actinomycin D （100μg/mL） caused only a 10% decrease in resveratrol content.

关 键 词：花生 种子 白藜露醇 黄曲霉菌 抗性 

分 类 号：S565.201[农业科学—作物学]