31份江苏省白菜型油菜遗传多样性的RAPD分析  

RAPD analysis for the genetic diversity of 31 B.campestris L from Jiangsu province in China

作　　者：陈碧云[1] 伍晓明[1] 许鲲[1] 王汉中[1] 李响枝[1] 

机构地区：[1]中国农业科学院油料作物研究所农业部油料作物遗传改良重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2006年第1期67-71,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金重点项目(39830270)

摘　　要：应用RAPD标记对31份来自江苏省白菜型油菜的遗传多样性进行了分析,选用20对随机引物扩增结果,共获得312个RAPD标记位点,其中205个位点具有多态性,多态率达65.7%。根据312个标记位点信息,用UPMGA法建立了江苏省31份白菜型油菜的亲缘关系聚类图,结果显示,在遗传距离0.0988上,31个品种分Ⅰ、Ⅱ两个大簇,第Ⅰ簇5个品种,第Ⅱ簇26个品种;在遗传距离0.0679上,簇Ⅱ分三个亚簇ⅡA、ⅡB、ⅡC,亚簇ⅡA 3个品种,亚簇ⅡB 21个品种,亚簇ⅡC 2个品种;来自南通县的南通黄油菜和淮阴县的清江大菜籽之间的遗传距离最小,为0.0577,亲缘关系最近。对来自同一县的多个品种的RAPD结果进行了遗传多样性指数分析,结果显示,SHANNON-WEAVER多样性指数和SIMPSON指数结果一致。31 B. campestris L DNA samples from Jiangsu province were amplified with 20 random primers, 312 RAPD alleles were obtained. There were 205 polymorphic alleles in the 312 RAPD alleles. The polymorphic rate was 65.7%. Using 312 marker alleles with UPMGA method, a dendrogram with 31 B. campestris L from Jiangsu province was constructed. Genetic distance among 31 varieties showed that the genetic distance between Nantong huangyoucai and Qingjiang dacaizi was the smallest, being 0. 0577. The relationship between Nantong huangyoucai and Qingjiang dacaizi was the closest. RAPD dendrogram was consisted of two groups. Group Ⅰ was made up of 5 varieties and Group Ⅱ was made up of 26 varieties. Genetic diversity index was analyzed using the RAPD band pattern. The result showed that the data of Shannon - Weaver index and Simpson index were consistent.

关 键 词：白菜型油菜 RAPD标记 遗传多样性 

分 类 号：S565.402.1[农业科学—作物学]