静脉免疫球蛋白对新生儿脐血淋巴细胞干扰素γ和白细胞介素4分泌的影响  

Effect of intravenous immunoglobulin on IFN-γ and IL-4 production of cord blood lymphocytes in neonates

作　　者：郝轶群[1] 陈同辛[1] 朱亚忠[1] 李庆生[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所免疫/肿瘤研究室,上海200092

出　　处：《中华围产医学杂志》2006年第2期112-116,共5页Chinese Journal of Perinatal Medicine

基　　金：国家自然科学基金资助项目(39600158)

摘　　要：目的研究静脉免疫球蛋白(intravenous immunoglobulin,IVIG)对新生儿淋巴细胞免疫功能的调节作用。方法分离8例新生儿的脐血单个核细胞和CD3+T细胞,并利用IVIG和植物血凝素(phytohemagglutinin,PHA)不同组合(即(1)对照组、(2)PHA刺激组、(3)IVIG先抑制组、 (4)PHA先活化组和(5)PHA+IVIG组)对细胞进行刺激培养,然后利用ELISA法检测细胞干扰素 (interferon,IFN)γ和白细胞介素(interleukin,IL)4的分泌,RT-CR法检测细胞IL-2 mRNA和IL-4 mRNA表达,并与8例成人外周血单个核细胞做比较。结果加入IVIG后,PHA诱导的脐血单个核细胞和CD3+T细胞IFN-γ的分泌下降,其中以单个核细胞下降的程度更为明显;并且单个核细胞IFN-γ分泌下降与IVIG加入的先后顺序无关PHA刺激组(313．34±108．00)pg／ml,先抑制组 (17．68±17．98)pg／ml,先活化组(170．72±38．25)pg／ml,PHA±IVIG组(11．10±8．92)pg／ml;但是CD3+T细胞IFN-γ的分泌下降只出现在先加入或同时加入IVIG时PHA刺激组(35．30±12．21) pg／ml,先抑制组(8．61±6．21)pg／ml,PHA±IVIG组(8．54±1．57)pg／ml。另外加入IVIG后,脐血单个核细胞IFN-γ分泌下降不如成人外周血单个核细胞明显,PHA刺激组(1121．11±344．58) pg／ml,先抑制组(29．08±11．20)pg／ml,先活化组(339．63±47．43)pg／ml,PHA±IVIG组(26．40± 21．97)pg／ml。单独加入PHA后,脐血单个核细胞和CD3+T细胞IL-4的分泌无明显变化,但是经过PMA、PHA和rIL-2反复刺激后脐血单个核细胞却能表达IL-2 mRNA和IL-4 mRNA,并且加入 IVIG后,脐血单个核细胞IL-2 mRNA和IL-4 mRNA表达明显下降。结论 IVIG除了可以直接抑制脐血T细胞分泌IFN-γ外,还可能通过其他免疫细胞或分子间接地介导这种抑制作用。IVIG 也可以抑制脐血单个核细胞IL-2 mRNA和IL-4 mRNA的表达。因此IVIG对脐血淋巴细胞增殖和 B细胞免疫球蛋白释放的影响可能与这些抑制作用有关。Objective To study the effects of intravenous immunoglobulin （IVIG） on neonatal lymphocyte immune function. Methods Cord blood mononuclear cells （CBMCs） and CD3^＋ T lymphocytes were isolated from 8 neonates and were cultured with IVIG and/or/without phytohemagglutinin（PHA）based on which divided into five groups, i.e. （1）control group, （2）PHA activation group, （3） IVIG pre-inhibition group, （4） PHA pre-activation group and （5） PHA＋IVIG group. The production of IFN-γ and IL-4 of CBMCs and cord blood CD3^＋ T lymphocytes was measured with ELISA and the expression of IL-2 and IL-4 mRNA was measured with RT-PCR. The results were compared with peripheral blood mononuclear cells（PBMCs） of 8 adults. Results The production of IFN-γ by CBMCs and cord blood CD3^＋ T lymphocytes induced by PHA decreased after IVIG was given, while more remarkable decrease in the CBMCs was shown without any association with IVIG was given or not before or after PHA was used [Group 2： （313.34±108.00）pg/ml, Group 3： （17.68±17.98） pg/ml, group 4 ： （170. 72±38. 25） pg/ml, group 5 ： （11.10±8.92）pg/ml]. For the CD3^＋ T lymphocytes, the decrease of IFN-γ production was only observed in group 3, 4 and group 5 [-（35.30 ±12.21）pg/ml, （8.61±6. 21） pg/ml and （8.54±1.57）pg/mll]. The degree of decrease of IFN-γ production by CBMCs was much lower Egroup 2：（1121.11 ± 344. 58） pg/ml, group 3： （29. 08 ± 11.20）pg/ml, group 4： （339.63±47.43） pg/ml, group 5： （26.40±21.97）pg/ml]. The secretion for IL-4 did not changed when stimulated by PHA alone, but IL-2 and IL-4 mRNAs expression can be detected in CBMCs after repeated stimulation by PMA, PHA and rIL-2 which decreased after IVIG was given. Conclusions IVIG can suppress the production of IFN-γ in cord blood T lymphocyte directly or mediated by other immune cells or molecules. IVIG can also decreased the expression of IL- 2 mRNA and IL-4 mRNA in CBMCs. The effect of IVIG on the proliferation of c

关 键 词：婴儿 新生 淋巴细胞 免疫球蛋白类 静脉内 干扰素Ⅱ型 白细胞介素4 

分 类 号：R722.1[医药卫生—儿科]