新生儿宫内感染乙型肝炎病毒后T淋巴细胞亚群的变化  

Change of T lymphocytic cell subgroups in infants after intrauterine infection with hepatitis B virus

作　　者：余敏敏[1] 韩国荣[1] 王建芳[1] 

机构地区：[1]东南大学医学院附属南京第二医院妇产科,江苏南京210003

出　　处：《中国实用妇科与产科杂志》2006年第5期364-366,共3页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的通过对宫内感染乙型肝炎病毒(HBV)新生儿T淋巴细胞亚群的检测,探讨宫内感染免疫失败的机制,为以后免疫干预提供理论依据。方法选择2003-03-2003-07在东南大学附属南京市第二医院分娩的90例HBsAg阳性孕妇的新生儿,实施母婴联合免疫,于注射乙型肝炎免疫球蛋白(HBIG)前抽取股静脉血用流式细胞仪检测T淋巴细胞亚群,采用酶联免疫法(ELISA)检测乙型肝炎病毒标志物(HBVm)定量。1个月龄时复查HBVm并根据HBsAg情况分为宫内感染组和非宫内感染组。比较两组婴儿血清中T淋巴细胞亚群CD3+、CD4+、CD8+的绝对计数和百分数。结果宫内感染组新生儿CD4+绝对计数和百分数均低于未感染组,而CD8+绝对计数和百分数均高于未感染组,CD4+/CD8+比值低于未感染组,两组间差异均有显著意义,P<0·05。CD3+绝对计数两组间差异无显著性意义,P>0·05。结论宫内感染HBV新生儿可能存在T淋巴细胞亚群比例失调,有可能通过增强特异性免疫反应而得到治疗。Objective To approach the mechanism of immunization failure in intrauterine infected infants with hepatitis B virus by detecting T lymphocytic cell subgroups so as to provide a theory foundation for immune intervention afterwards. Methods We selected 90 infants born by HBsAg positive mothers in the Second Affiliated Hospital of Nanjing of Southeast Medical University from May to July in 2003. T Lymphotcytic cell subgroups were measured by using flow-cytometry, and Hepatitis B virus mark were detected by using ELISA in all blood samples taken from femoral vein of all infants before hepatitis B immunoglobulin injected. All the infants had re-examination for HBVm at 1 month old and were divided into intrauterine infection group and non-intrauterine infection group accgrding to their HBsAg behavior. T lymphotcytic cell subgroups count and percentage of CD3 ＋ ,CD4 ^＋ ,CDs ＋ were compared between the two groups. Results The count and percentage of CD4 ^＋ T cell and the ratio of CD4 ^＋/CD8 ^＋ T cell were lower while the CD8 ^＋ T cell,count and percentage were higher in the intrauterine infection group than those in the control group. The difference of 2 groups was significant, P 〈 0.05 . The counts of CD3 ^＋ T cell in 2 groups had no significant difference, P 〉 0.05. Conclusion The intrauterine infection with HBV may be related to lopsided development of T lymphocytic cell subgroups and intrauterine infection infants may be treated by reinforcing specific immunologic reaction.

关 键 词：肝炎病毒 乙型 宫内感染 T淋巴细胞亚群 

分 类 号：R71[医药卫生—妇产科学]