托瑞米芬对更年期大鼠骨与子宫组织的选择性调节作用  

Elective modulation of toremifene on bone and uterine tissues of menopausal rats

作　　者：夏贤[1] 张绍芬[1] 刘旸[2] 蒋竞[1] 朱慧庭[1] 毕崇萍[3] 刘建华[4] 

机构地区：[1]复旦大学附属妇产科医院,上海200011 [2]南京市鼓楼医院 [3]上海市华东医院 [4]中科院上海药物所毒理组

出　　处：《中国骨质疏松杂志》2006年第2期124-127,共4页Chinese Journal of Osteoporosis

基　　金：上海市科委基础研究重点项目(04JC1492);上海市医学重点学科(妇产科学)建设项目(05Ⅲ0165)

摘　　要：目的了解托瑞米芬对更年期大鼠骨与子宫组织的选择性调节作用。方法30只SD雌性大鼠(12m龄)随机分为更年组(生理盐水灌胃每只1mLd),雌激素组予戊酸雌二醇800μgkg·d灌胃,托瑞米芬组予6mgkg·d灌胃。3个月后处死动物,测定血清E2水平、称子宫湿重、DPXL型双能X线骨密度仪测定腰椎与股骨骨密度,IMS细胞自动图像分析系统测定子宫腔上皮厚度(μm)、子宫间质厚度(μm)、子宫横切面面积(mm2)进行计量分析,RTPCR技术测定子宫内膜ERαmRNA表达。结果托瑞米芬组子宫湿重、子宫(除腔上皮以外)的各计量参数、子宫内膜ERαmRNA表达水平均明显低于雌激素组和更年组;而腰椎与股骨骨密度值与雌激素组无明显差异,均大于更年组。结论托瑞米芬对更年期大鼠骨组织呈类雌激素样作用,对子宫及内膜组织呈抗雌激素作用。其抗雌激素效应与ERαmRNA表达下降有关,并与机体激素环境有关。Objective To investigate the selective modulation of toremifene on the bone and uterine tissue of menopausal rats.Methods Thirty female SD rats（ 12-months old） were randomized into three groups：menopause group （fed with distilled water t mL/d） ;estrogen group （fed with estradiol valerate 800/lg/kg＂ d） g toremifene group （fed with toremifene 6 mg/kg＂ d）. All rats were sacrificed 3 months later, the level of serum estradiol（E2 ） was determined and the gross weight of uterus were scaled. The lumbar and femoral bone mass density （BMD） were measured with the DEXA Bone Densitomor and the histologic parameters of the uterus were analyzed, including luminal epithelial thickness（/~m）, stromal thickness（lzm）, and cross-sectional area（mm~） with IMS automatic cell- image-αnalyzing system, and the expression of endometrial ER-α mRNA was determined by reverse transcription- polymerase chain reaction（ RT-PCR）. Results The gross weight and histologic parameters of the uterus except luminal epithelial thickness and the ER-α mRNA expression in toremifene group were significantly lower than those of the other two groups. No significant difference was found between the toremifene group and the estrogen group in the lumbar and femoral BMD. The lowest BMD was found in the menopause group. Conclusions Toremifene has estrogen-like effect on the bone tissue and antl-estrogen effect on the uterus and endometrium in menopausal rats. The anti-estrogen effect is related with the decreased ER-α mRNA expression and hormonal level of the body.

关 键 词：托瑞米芬 雌激索 子宫内膜 ER-Α mRNA:BMD 

分 类 号：R811.5[医药卫生—放射医学] R285.5[医药卫生—临床医学]