高效液相色谱法测定续断药材中川续断皂苷Ⅵ的含量  

Determination of akebia saponin D in root of Dipacus asperoides by HPLC

作　　者：谭洪根[1] 林生[1] 张启伟[1] 吉力[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2006年第9期726-727,739,共3页China Journal of Chinese Materia Medica

基　　金：国家重点科技攻关计划项目(99-929-01-034)

摘　　要：目的：建立中药续断中川续断皂苷Ⅵ的含量测定方法，为续断质量标准建立提供依据。方法：采用反相高效液相色谱法，Kromasil ODS色谱柱，流动相为乙腈-水（30：70），检测波长为212nm。结果：续断中川续断皂苷Ⅵ在该色谱条件下获良好分离，线性范围为0．5825-9．32μg，r=0．9999，平均加样回收率为100．3％，重复性RSD为2．3％。结论：该方法方便，快速，准确，可作为续断药材及其制剂质量控制的方法。Objective： To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides. Method： An HPLC analytical method was established using Kromasil ODS column with acetonitrile-water （30：70） as mobile phase and the detection wavelength was 212 nm. Result： The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 μg, r = 0.999 9. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively. Conclusion： This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.

关 键 词：续断 川续断皂苷Ⅵ HPLC 

分 类 号：R284[医药卫生—中药学]