双黄升白颗粒剂对白细胞减少症模型小鼠造血细胞作用的研究  

Experimental research of Shuanghuang Shengbai granule on hematopoietic cells of leucopenia model mice

作　　者：沈云辉[1] 陈长勋[1] 徐振晔[2] 

机构地区：[1]上海中医药大学药理教研室,上海201203 [2]上海中医药大学附属龙华医院,上海200032

出　　处：《中国中药杂志》2006年第9期754-759,共6页China Journal of Chinese Materia Medica

基　　金：上海市科学技术委员会资助项目(02DZ19136)

摘　　要：目的:探讨双黄升白颗粒剂升高白细胞作用及其作用机制。方法:采用Se-137放射源照射ICR小鼠,造成白细胞减少症动物模型,对模型动物给予双黄升白颗粒剂大、中、小剂量(分别为9.2,4.6,2.3 g.kg-1.d-1)灌胃,并设正常对照组,模型对照组,阳性对照利可君组。对小鼠外周血象,骨髓有核细胞(BMNC),脾结节(CFU-S)计数,胸腺指数(TI),脾脏指数(SI),骨髓细胞增殖,体外培养小鼠粒单系集落形成单位(CFU-GM),骨髓CD34+细胞比例,骨髓造血微环境等进行检测。结果:双黄升白颗粒可明显升高模型小鼠的白细胞(WBC);双黄升白颗粒可升高模型小鼠的BMNC计数并促进其骨髓细胞增殖;双黄升白颗粒升高模型小鼠CFU-S计数,提高CFU-GM产率,提高骨髓CD34+细胞比例,对造血干/祖细胞增殖具促进作用;双黄升白颗粒具有保护模型小鼠骨髓造血微环境作用;双黄升白颗粒升高模型动物SI,具有一定的免疫增强作用。结论:双黄升白颗粒剂具有明显升高白细胞作用,其作用机制与促进造血细胞增殖及保护骨髓造血微环境有关。Objective： To investigate the leukogenic function of Shuanghuang Shengbai （SHSB）granule and the related mechanisms. Method： Mouse leukopenic models were induced by radiation. Mice were divided into normal control group, model control group, positive control group-Li kejun tablet group and three different dose （high, middle, low-dose） groups of SHSB granule. The peripheral hemograrn, thymus index（TI）, spleen index（SI）, bone marrow nucleated cell （BMNC） and colony forming unit-spleen （CFU-S） were evaluated. The proliferation of bone marrow cells was determined. The in vitro cultured colony forming unit granulocyte macrephage（CFU-GM） was estimated. The index of CD34^＋ cell in BMNC were determined by flow cytometry. The ultra-micro structure of bone marrow were observed by electromicrescope. Result： （1）SHSB rranule could increase the WBC of model mice; （2）SHSB granule could increase BMNC and promote the proliferation of bone marrow cell; （3）SHSB granule could increase CFU-S, CFU-GM and CD34^＋ cell index in BMNC of model mice significantly;（4）SHSB Granule could also protect the bone marrow hemotopoietic from the harm of radiation; （5）SHSB granule could increase the SI of model mice, indicating the enhancement of immunological function. Conclusion： SHSB granule has apparent leukogenic function. The mechanism may be related to enhancing the proliferation of hematopoietie cells and protecting the bone marrow hemotopoietic miereenvimnment.

关 键 词：双黄升白颗粒剂 白细胞减少症 升高白细胞作用 造血细胞增殖 造血微环境 

分 类 号：R285[医药卫生—中药学]