分光光度法测定浮游植物叶绿素a的比较研究  

Comparison of Measurement of Phytoplankton Chlorophyll-a Concentration by Spectrophotometry

作　　者：李振国[1] 卢军[2] 王国祥[2] 葛绪广[1] 

机构地区：[1]南京师范大学地理科学学院,江苏南京210097 [2]南京农业大学资源与环境科学学院,江苏南京210095

出　　处：《中国环境监测》2006年第2期21-23,共3页Environmental Monitoring in China

基　　金：教育部"211工程"重点学科建设项目;江苏省科技项目

摘　　要：对浮游植物叶绿素a测定方法中的国家标准方法丙酮三色法与目前国际上使用的方法乙醇单色法进性了比较,实验材料使用了实验室培养的栅藻,避免了藻类种类对于测定结果的影响。实验结果显示,乙醇法的萃取率高于丙酮法,但由于乙醇法实验条件的难以控制,其结果的方差较大;两种方法的测定结果间的相关性很好,相关系数为0.9998,因此得出两种方法测定结果间的换算公式为Chla乙醇=1.658 Chla丙酮-5.821。还研究了乙酸滤膜的溶解对于测定结果的影响,结果表明乙酸滤膜的溶解未对测定结果造成明显的影响。Chlorophyll-a content is widely used as an indicator of the quality of freshwater bodies. Chlorophyll-a contents of cultures of the chlorophyte Sencedesmus subspicatus determined by means of the 90% hot ethanol and the 90% acetone in this study, And the results of the two methods were compared. The results show that the hot ethanol method has more complete extraction, but the acetone method is easier to handle. By using the formula Chlaethanol= 1.658 Chlaacetone - 5.821, the acetone method data can be transferred to the hot ethanol method data. And the results show that the interferer of acetic acid fiber filters＇ dissolving to the results of Chlorophyll- a contents is minuteness,

关 键 词：叶绿素A 丙酮萃取法 热乙醇萃取法 

分 类 号：X832[环境科学与工程—环境工程]