L-精氨酸对果糖喂养大鼠血压及胰岛素抵抗影响的实验研究  

Effects of L-arginine on blood pressure and insulin-resistance in fructose-fed rats

作　　者：刘庆[1] 李玉明[2] 刘惠亮[1] 

机构地区：[1]武警总医院心血管内科,北京100039 [2]武警医学院附属医院,天津300162

出　　处：《武警医学》2006年第4期260-263,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：天津市自然科学基金重点项目(NO.023803611)

摘　　要：目的探讨L-精氨酸是否能够防止大鼠胰岛素敏感性的下降及血压的升高.方法本研究通过观察L-精氨酸（L-Arg）对果糖喂养胰岛素抵抗大鼠血压、胰岛素抵抗的影响,5～6周龄的雄性SD大鼠,适应性喂养1周后随机分为4组.果糖组（FFR）喂以60%的高果糖饲料,对照组（Contro1）喂以热量相同的普通饲料,果糖＋I-精氨酸（FFR＋L-Arg）组给予高果糖饲料喂养的同时饮水中加入L-精氨酸（浓度2.25%）,L-精氨酸（L-Arg）组给予普通饲料喂养的同时饮水中加入L-精氨酸.每周鼠尾测血压1次、每两周断尾取血查血清胰岛素（FINS）、血糖（FSG）;6周后处死大鼠,腹主动脉取血,测定血清胰岛素（FINS）、血糖（FBG）、血三酰甘油（TG）.结果高果糖喂养6周后大鼠出现高胰岛素、高三酰甘油血症并伴有血压的升高;FFR＋L-Arg组FINS、TG均较FFR组明显降低;FFR＋L-Arg组在整个实验过程中血压不出现升高.结论高果糖喂养可以使正常SD大鼠出现胰岛素抵抗并伴有血压升高、血脂代谢紊乱;而L-精氨酸可以改善胰岛素抵抗及血脂代谢紊乱,并防止大鼠出现血压升高.Objective To study whether L- arginine prevents elevated blood pressure and improves insulin resiatance in fructose - fed rats. Methods Adaptively fed for one week, male Sprague- Dawley rats aged 5 - 6 weeks were divided into four groups：fructose （FFR） group fed with 60% high fructose - diet;control group fed with regular diet with the same calories; fructese ＋ L - arginine （FFR ＋ L- Arg） group fed with high fructose - diet and 2.25% L- arginine solution;and L- arginine（L- Arg） group fed with regular diet and 2.25% L- argmine solution. Blood pressure was measured weekly; serum insulin and glucose were assayed biweekly. Six weeks later, all the rats were sacrificed and their blood samples were collected; the concentrations of fasting serum insulin （FINS） and triglyceride（TG） were determined. Results FFR group had marked hyperinsulinemia,hypertension, hypertriglyceridemia （ P 〈 0.05）. Serum insulin and triglyceride levels in FFR ＋ L - Arg group were significantly lower than those in FFR group （ P 〈 0.05）, and no hypertension appeared in FFR ＋ L - Arg. Conclusions High fructose feeding results in insulin resistance accompanied by elevated blood pressure and dyslipidemia. L - Arginine can prevent hypertension and improve insulin resistance and dyslipidemia.

关 键 词：胰岛素抵抗 果糖 高血压 L-精氨酸 

分 类 号：R977.4[医药卫生—药品] R587.1[医药卫生—药学]