熊猴实验感染人乙型肝炎病毒的初步研究  

EXPERIMENTAL INFECTION OF MACACA ASSMENSIS BY HUMAN HEPATITIS B VIRUS ( HBV )

作　　者：葛宪民[1] 陈杰[1] 黄果勇[1] 黄达春[1] 刘伟[1] 蒋云志 李荣健[1] 王树声[1] 

机构地区：[1]广西壮族自治区卫生防疫站

出　　处：《病毒学报》1990年第1期19-26,共8页Chinese Journal of Virology

基　　金：广西卫生厅青年科学研究基金

摘　　要：选用7只无人乙型肝炎病毒(HBV)自然感染的熊猴,经下肢静脉接种人急性或慢性乙型肝炎患者血清,每 2周采血1次,检测肝功能,同时用RPHA法、ELISA法、SPRIA法及斑点杂交法检测各项血清学HBV标记,并通过肝穿刺活检进行光学显微镜和电镜病理学检查,以及用组织化学、免疫组织化学和原位杂交等法检测肝细胞内HBsAg、HBcAg和HBV—DNA.结果,7只熊猴的血清SGPT、HBsAg、抗-HBs、抗-HBc和HBV—DNA均有阳性,所有实验猴的肝组织病理学改变均与急性乙型肝炎病人的肝脏病变相同,并可在有的猴肝细胞内找到HBsAg和HBV-DNA。Seven normal Macaco assnensis were inoculated with serum of patients with either acute or chronic hepatitis B. After inoculation, the liver function test and serology of HBV by RPHA, ELISA, SPRIA. and hybridization spot test were examined. The biopsied liver specimens were examined by histopathology and by electron microscopy, and for intrahepatic HBsAg, HBcAg and HBV-DNA. The results showed that the animals were positive for SGPT, HBsAg, anti-HBs, anti-HBc and HBV-DNA, the liver pathology agrees with that of patients of acute hepatitis B. In some biopsied monkey, HBsAg and HBV-DNA were demonstrated in the hepato-cytes.

关 键 词：熊猴 实验感染 乙型肝炎病毒 

分 类 号：R373.21[医药卫生—病原生物学]