从肝癌组织中发现乙型肝炎病毒核心抗原编码基因突变  

DETECTION OF HEPATITIS B VIRUS C GENE VARIATION IN HEPATOCELLULAR CARCINOMA

作　　者：陈子平[1] 闻玉梅[1] 顾健人[2] 范大方 陈渊卿[2] 严根宝[2] 

机构地区：[1]上海医科大学微生物学教研室 [2]上海市肿瘤研究所

出　　处：《病毒学报》1990年第2期192-195,共4页Chinese Journal of Virology

摘　　要：乙型肝炎病毒(HBV)引起的持续性感染是临床及流行病学的一大问题。导致HBV持续感染的因素之一可能是HBV出现变异株。我们曾以HBV表面抗原(S)基因、核心抗原(C)基因与乙型肝炎患者肝组织做核酸分子杂交,发现少数患者肝内C基因杂交信号减弱,1例有S基因整合但C基因缺失。为进一步研究C基因的变化,将33例原发性肝癌组织DNA,分别与C基因杂交。结果14/33的S抗原阳性,但其中仅3/14为C基因阳性,反映大多数肝癌患者C基因缺失。Southern吸印转移后杂交显示,3例肝癌组织中,2例同时存在复制型与整合型C基因(HBC1,HBC3),1例仅有复制型C基因(HBC2)。见图1。Thirty three hepatocellular carcinoma tissues were hybridized separately with hepatitis B virus ( HBV ) surface ( S ) and core ( C ) genes. Fourteen out of 33 were S positive, whereas only 3/14 were C gene positive. These 3C gene positive liver cancer tissues were amplified for C( including Pre-C gene)gene by polymerase chain reaction,further cloned and sequenced. Comparing with the HBV adr subtype clone ( pADR-1 ) nucleotide sequence, there were point mutations in all 3 clones, including transition and trans-version but no frame shift. There was a shared point mutation at the 1,898 nucleotide sequence in 2 clones, which was a transition from guanine to adenine,leading to a stop codon in the pre-C region, 2 codons upstream from the ATG of C gene. Since one of the clones was isolated from a sample which revealed only replicating form of C gene,an HBV C gene variant was implied. Although most point mutations were neutral, some amino acid alterations were present,hut no regularity of alterations was detected. Studies on the potential biological significance of this variant are being pursued.

关 键 词：肝癌 乙型肝炎病毒 核心抗原 

分 类 号：R373.21[医药卫生—病原生物学]