大菜粉蝶颗粒体病毒颗粒体蛋白基因的定位  

STUDIES ON LOCATION OF THE GRANULIN GENE FROM PIERIS BRASSICAE GRANULOSIS VIRUS ( PbGV )

作　　者：赵民安[1] 刘云英[1] 杨向明[1] 吾尔尼沙[1] 李维琪[1] 

机构地区：[1]中国科学院新疆化学所

出　　处：《病毒学报》1990年第3期277-279,共3页Chinese Journal of Virology

摘　　要：在昆虫杆状病毒颗粒体蛋白基因的定位研究中,用颗粒体蛋白mRNA来定位其基因,国内工作较少。本文报道用大菜粉蝶颗粒体病毒(Pieris brassicae granulosis virus,Pb-GV)颗粒体蛋白的mRNA对相应基因进行定位的结果。Restricted fragments of PbGV-DNA digested with XhoI and EcoR I were labeled with α-[22P] dATP. The results of hybridization of poly ( A ) RNA to restricted fragments indicated that the granulin gene of Pieris brassicae granulosis virus is in Xho I-I and EcoR I-V fragments.When use T.ni granulin probe the results of hybridization of the probe to restricted fragments digested with EcoR I confirmed our conclusion.

关 键 词：PbGV 基因定位 颗粒体蛋白 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]