乙型肝炎病毒基因型与HBV宫内传播关系的研究  

STUDY ON THE RELATIONSHIP BETWEEN HBV GENOTYPE AND INTRAUTERINE TRANSMISSION.

作　　者：李淑珍[1] 王素萍[2] 双杰玉[3] 冯丽萍[3] 

机构地区：[1]山西医科大学第一医院,太原030001 [2]山西医科大学流行病学教研室 [3]太原市传染病院妇产科

出　　处：《现代预防医学》2006年第5期660-661,共2页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30070699)

摘　　要：目的:探讨乙型肝炎病毒基因型与HBV宫内传播之间的关系。方法:采集170例HBsAg阳性孕妇及其172例新生儿外周血,巢式PCR检测血清HBV DNA,PCR-RFLP技术结合PCR产物直接测序鉴定HBV基因型。结果:太原地区HBsAg阳性孕妇及其新生儿携带乙型肝炎病毒有B、C、D 3种基因型,以C型为优势基因型。HBV C型与B型孕妇HBV宫内感染率分别为56.45%、50.00%(χ2=0.092,P=0.761),两组孕妇的HBV宫内感染率差异无统计学意义。所有发生宫内感染的新生儿HBV基因型均与其母亲一致。结论:孕妇感染HBV C型与B型其新生儿发生HBV宫内感染的几率相当。乙型肝炎病毒宫内传播过程中,HBV基因型可能不发生改变。Objective： To explore the relationship between hepatitis B virus （HBV） genotypes and intrauterine transmission. Methods： 170 HBsAg positive pregnant women and their newborns＇ sera samples were collected. HBV genotypes were determied by nested- polymerase chain reaction and restriction fragment length polymorphism （nPCR - RFLP） analysis combined with PCR - directed nudeotide sequencing. Results： There were three genotypes - B, C and D in these pregnant women and newborns. Genotype C was the major genotype, The rate of HBV intrauterine infection in pregnant women who carries HBV C and B were 56.45% and 50.00% respectivdy （ X^2 = 0,092, P = 0.761）. There was no significant difference between them. HBV genotypes of all newboms were same with that of their mothers＇. Conclusion： HBV intrauterine infection rate in newborns was not different between pregnant women carried HBV C and B. HBV genotypes would not change in intrauterine transmission.

关 键 词：肝炎病毒 乙型 基因型 疾病传播 垂直 

分 类 号：R512.62[医药卫生—内科学]