应用荧光蛋白磷酸酶抑制法检测水中微囊藻毒素  

A FLUORESCENT PROTEIN PHOSPHATASE INHIBITION ASSAY FOR THE DETECTION OF MICROCYSTINS IN WATER.

作　　者：王亚[1] 尹立红[1] 浦跃朴[1] 张立将[1] 朱光灿[2] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]东南大学土木工程学院

出　　处：《现代预防医学》2006年第5期681-683,共3页Modern Preventive Medicine

基　　金：国家高技术研究发展计划资助项目(2002AA601011);江苏省自然科学基金(BK2005068)

摘　　要：目的：以DiFMUP为底物，建立微囊藻毒素的荧光蛋白磷酸酶抑制检测法（F-PPIA）并探讨其在水体微囊藻毒素检测中的应用。方法：对太湖水华期间水样分别应用F-PPIA和HPLC方法检测微囊藻毒素含量，并进行相关性分析；应用F-PPIA方法检测南京玄武湖、莫愁湖、南湖等富营养化水体中微囊藻毒素含量，同时应用丙酮提取-分光光度法检测水体中叶绿素含量。结果：F-PPIA法对水中MC的检测范围是（0．02～0．5）μg／L。FPPIA和HPLC方法检测水样中微囊藻毒素物质的结果相关性较好（r=0．9678，P〈0．001）。南京玄武湖、莫愁湖、南湖水体中微囊藻毒素类物质分别为（6．671～14．579）、（0．096～0．166）、（0．050～0．057）μg／L，叶绿素含量分别为（51．182～183．956）、（40．946～65．513）及40．946μg／L。结论：F-PPIA法能快速、准确检测环境样本中微囊藻毒素含量，具有一定的实用价值。南京7～8月玄武湖水体的富营养化程度和微囊藻毒素类物质污染程度较莫愁湖、南湖严重。Objective： To establish fluorescent protein phosphatase inhibition assay （F- PPIA） for microcystins （MC） based on DiFMUP as substrate and explore the application of F- PPIA in the detection of MC in water.Method： MC in Tai lake was detected by HPLC and F- PPIA, respectively. And correlation was analyzed between two groups of detecting results. MC and chl a which in Xuanwu Lake, Moehou Lake and Nanhu Lake were measured by F- PPIA and spectrophotometer.Result： The results showed that the detecting range of F - PPIA was 0.02～0.5μg/L and the corrdation between F - PPIA and HPLC detection results was good （r = 0. 9438, P〈0.001） . The contents of MC of Xuanwu Lake, Mochou Lake and Nanhu Lake were （6. 671 ～ 14. 579）, （0.096～0.166） and （0.050～0.057） μg/L respectivdy; and contents of chl- a were（51.182～183.956）, （40.946～65. 513） and 40.946μg/L respectively.Conclusion： F- PPIA is a rapid, sensitive method, which is practical for screening and monitoring a large number of water samples containing low concentration of MC.And during July to August, pollution of MC in Xuanwu Lake was severer than that of Moehou Lake and Nanhu Lake.

关 键 词：微囊藻毒素 荧光蛋白磷酸酶抑制法 水体污染 高效液相色谱法 

分 类 号：R123.1[医药卫生—环境卫生学]