蒙古黄芪异黄酮的测定与提纯工艺研究  

Studies on determination and purification technology of isoflavonesin astragalus mongholicus

作　　者：符敬伟[1] 郭海波[2] 乔卫[1] 周晶[1] 

机构地区：[1]天津医科大学药学院,天津300070 [2]北京铁营医院,北京100075

出　　处：《天津药学》2006年第2期19-20,共2页Tianjin Pharmacy

摘　　要：目的:测定蒙古黄芪异黄酮的含量,并考查用大孔吸附树脂分离纯化异黄酮的方法。方法:本试验采用甲醇回流提取黄芪异黄酮,于262 nm波长处测定异黄酮的含量。结果:测得总异黄酮含量为1.34 mg/g,RSD为1.18%(n=3),加样回收率为99.25%,RSD为1.93%(n=6)。结论:采用直接比色法测量异黄酮是可行的;确定以NKA-12型大孔吸附树脂富集黄芪异黄酮比较合适。Object： To determine the content of isoflavones in Astragalus monghohcus Bunge and to investigate the method for separating and purifying isoflavones on the macro - reticular resin. Methods： The isoflavones were extracted with methanol and determined at 262 run. Results： The content of isoflavones was 1.34 mg/g, and RSD was 1.18 % （ n = 3 ）. The recovery was 99.25 % with RSD of 1.93 % （ n = 6）. Conclusion： The method was simple and accurate. The NKA - 12 type macro - reticular resin was suitable for gathering isoflavones.

关 键 词：黄芪 异黄酮 含量测定 大孔吸附树脂 

分 类 号：R927.2[医药卫生—药学]