9种胡枝子亲缘关系的ISSR分析  

Inter-simple Sequence Repeat (ISSR) Analysis on genetic relationship in Lespedeza spp.

作　　者：赵杨[1] 陈晓阳[1] 王秀荣 孙文生[1] 

机构地区：[1]北京林业大学林木花卉遗传育种实验室,北京100083 [2]河南林业学校,河南洛阳471000

出　　处：《吉林林业科技》2006年第2期1-4,共4页Journal of Jilin Forestry Science and Technology

基　　金：国家高技术研究发展计划(863计划)"豆科饲料型灌木抗逆新品种选育"(2002AA241111)课题资助

摘　　要：利用ISSR-PCR方法对9种胡枝子的种间变异进行了研究,从60条引物中筛选出12条能扩增出清晰带型并具多态性的引物,共扩增出202个DNA片段,其中198个片段呈现多态性,占总扩增片段的98%。依据扩增结果进行遗传距离分析,构建了系统树。结果表明:9种胡枝子可以聚为4类,与形态学分类结果并不完全一致。Inter-species relationship of 9 species in Lespedeza was studied by using of ISSR marker. 12 primers were screened out from 60 random primers. Total 202 DNA bands were amplified 198 of which were polymorphic accounted for 98% ）. The average number of DNA bands amplified by each primer was 16.8. A tree diagram was constructed by using UPGMA algorithm. The 9 Lespedera species were classified into 4 groups, which is not consistent with the morphological classification.

关 键 词：胡枝子属 ISSR 亲缘关系 

分 类 号：Q523.8[生物学—生物化学]