用光生物素核酸探针检测牛传染性鼻气管炎病毒  

DETECTION OF INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS USING PHOTOBIOTIN LABELLED NUCLEIC ACID PROBE

作　　者：吴时友[1] 封启民[1] 蒋正军[1] 孙书华[1] 宫云浩[1] 李昌琳[1] 张瑞珍[1] 

机构地区：[1]农业部动物检疫所

出　　处：《病毒学报》1990年第4期363-367,共5页Chinese Journal of Virology

摘　　要：用光生物素标记牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitisvirus,IBRV)9.6kb DNA片段做探针,可检测出10pg的IBRV DNA、1个TCID50的病毒和1～10个TCID50的实验感染牛精液。用核酸探针检测IBRV与分离病毒的符合率为84.4％。若将采集的样品在牛肾细胞上增毒一次,再抽提培养物的核酸,用核酸探针检测,则敏感性和符合率大为提高。In this paper we report tie result of detection in infectious bovine rhinotracheitis virus ( IBRV ) using photobiotin labelled nucleic acid probe. The result showed that 10pg of IBRY DNA, 1 TCID50 of virus aad 1-10 TCID50 of experimentally contaminated bovine semen can be detected by using photobiotin labelled IBRV 9.6kb DNA fragment as probe. A comparison of detection of IBRV between nucleic acid probe and virus isolation indicates 84.4% agreement.

关 键 词：牛病毒 鼻气管炎病毒 光生物素 

分 类 号：S852.653[农业科学—基础兽医学]