单克隆抗体间接免疫荧光法检测巨细胞病毒分离培养中的抗原  

Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody

作　　者：段艳平[1] 陈秀珠[1] 吴远明[2] 

机构地区：[1]同济医科大学微生物学教研室,武汉430022 [2]中国科学院武汉病毒研究所,武汉430071

出　　处：《病毒学杂志》1990年第1期36-40,共5页

摘　　要：应用抗巨细胞病毒(HCMV)蛋白抗原(分子量为20千道尔顿)的单克隆抗体(McAb-20k)和HCMV IgG特异性阳性血清以间接免疫荧光试验检测28例器官移植病人尿标本接种人胚肺细胞后的HCMV感染情况,结果前法于接种后48小时检测到HCMV阳性病人9例,后者于接种6天检测到阳性病人11例。与病毒分离结果相比较,两法的敏感性分别为81.8％和90.9％,特异性相应为100％和94.12％,符合率均为92.9％,比病毒分离提前数天至数周作出诊断,重复性良好。因此,抗HCMV蛋白抗原的单克隆抗体间接免疫荧光法是一种有效的、早期快速而又敏感特异的诊断方法,操作简便,既使没有单抗,可用HCMV IgG阳性血清代替,也可取得较好效果,值得在一般实验室推广应用。Two indirect immunofluorescent assays(IIFA), using a monoclonal antibody directed against protein antigen (McAb-20k)and specific positive sera of human cytomegalovirus(HCMV)respectively, were applied for the rapid detection of the virus from fifty urine specimens(28 cases)inoculated onto human embryonic fibroblast. By the former procedure g of 28 cases were positive at 48 hours postinfection and all of them developed cytopathic effect; by the latter 11 were positive at 6 days postinfection and only 10 cases developed recognizable cytopathic effect. Comparing with the conventional cell culture in which 11 of 28 cases produced the cytomegalovirus cytopathic effect at a mean of 29-days after inoculation, the sensitivity was 81.8％ and 90.9％ respectively, the specificity was 100％ and 94.12％ respectively, and the coincidentity was both 92.9％. So, both procedures were effective, rapid, sensitive and specific. Using the McAh-20k could make a very early diagnosis and have significant practical value. But if there is no McAb, specifical positive sera could be substituted.

关 键 词：巨细胞病毒 抗原 单克隆抗体 检测 

分 类 号：R446.62[医药卫生—诊断学]