抗纤Ⅰ号和硒药物血清对肝星状细胞增殖和凋亡的影响  

Sera Pharmacological Effect of Anti-fibrosis Herbs and Selenium on the Proliferation and Apoptosis of Hepatic Stellate Cells

作　　者：贺宇彤[1] 刘殿武[2] 李青[2] 单保恩[1] 

机构地区：[1]河北医科大学第四医院,石家庄050011 [2]河北医科大学流行病教研室,石家庄050017

出　　处：《中华中医药杂志》2006年第4期219-222,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河北省自然科学基金资助项目(编号:302489)

摘　　要：目的:观察抗纤Ⅰ号(AF)和抗纤Ⅰ号加硒(AS)药物血清诱导大鼠肝星状细胞(HSCs)增殖、凋亡的作用,并探讨其作用的分子机制。方法:采用整体动物体内给药,分离制备含药血清,采用MTT法检测药物血清对HSCs增殖的影响;流式细胞仪检测细胞周期;Annexin-V/PI联合标记法测定细胞凋亡率;RT-PCR测定HSCs转化生长因子β1(TGF-β1)mRNA表达;电泳迁移率改变分析(EMSA)检测核转录因子κB(NF-κB)与DNA的结合活性。结果:AF和AS含药血清呈剂量依赖性抑制HSCs增殖,并可诱导HSCs凋亡,凋亡率(%)分别为18.07±2.67,17.55±1.91;两种含药血清可显著降低HSCs TGF-β1mRNA表达,抑制HSCs NF-κB的结合活性。结论:抗纤Ⅰ号和硒药物血清可以通过抑制HSCs的增殖、诱导HSCs凋亡,而使细胞外基质合成减少,肝纤维化减轻,其可能的分子机制是抑制NF-κB的活性,进而降低TGF-β1的表达。Objective： To investigate the sera pharmacological effect of anti-fibrosis herbs and selenium on the proliferation and apoptosis of hepatic stellate cells. Methods： The sera from the rats fed by colchicines （Col group）, anti-fibrosis herbs （AF group） and anti-fibrosis herbs plus selenium （AS group） acted on the hepatic stellate cells （HSCs）. MTT assays and Flow Cytometry were employed to estimate the proliferation of HSCs. The expressions of TGF-β1 mRNA were detected by semi-quantified RT-PCR. The activity of NF-κB binding DNA was examined by EMSA. Results ： The inhibitive rate of AF group and AS group were much higher than that of Col sera at the concentration of 10% -40%. The proliferation indexof AF group and AS group was lower than that of Col group statistically. Sera of treatment group could induce the apoptosis of HSCs. The expressions of TGF-β, mRNA in AF and AS group were markedly decreased compared with Col group. The activity of NF-κB binding DNA of AF group , AS group was much lower than that of Col group. Conclusion： These results suggested that anti-fibrosis herbs and selenium may inhibited the proliferation of HSCs and induce apoptosis of HSCs in vitro, which may be related to down-regulation the activity of NF-κB binding DNA and TGF-β, expression.

关 键 词：肝星状细胞 中药复方 硒 转化生长因子Β1 核转录因子ΚB 

分 类 号：R285.5[医药卫生—中药学] R733.71[医药卫生—中医学]