极谱免疫法测定抗血清中溶血素的活性  

Determination of Hemolysin Activity in Antiserum by Polarographic Immunoassay

作　　者：亢晓峰[1] 宋俊峰[2] 胡荫华[2] 

机构地区：[1]宝鸡文理学院化学化工系,宝鸡721007 [2]西北大学电分析化学研究所,西安710069

出　　处：《分析化学》1996年第6期626-629,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金;陕西省教委专项科学基金

摘　　要：本文报道一种测定溶血素活性的新方法。利用免疫溶血反应释放出的血红蛋白来催化邻苯二胺和H2O2的反应，通过酶促产物2，2＇-二氨基偶氮苯的极谱检测来确定溶血素的活性。测定范围为0．02～4.50U／mL，检测下限为0．018U／mL；一次可分析25份血样，已用于抗血清样品中溶血素活性的测定。A new method for determination of hemolysin activity in antiserum by polarographic immunoassay was reported in this paper. This method is based on the reaction between o-phenylenediamine and hydrogen peroxide catalyzed by hemoglobin released from cells in immune hemolysis, producing an electroactive compound 2, 2' -diaminoazobezene which is determined by linear-sweep polarography.The linear range is 0. 02 ~ 4. 5 U/mL with a detection limit of 0. 018 U/mL.The proposed method has been applied to the determination of hemolysin activity in antiserum samples.

关 键 词：溶血素 血红蛋白 极谱法 免疫分析 

分 类 号：R979.5[医药卫生—药品] O657.14[医药卫生—药学]