变色酸光度法测定海因  

Spectrophotometric Determination of Hydantoin With Chromotropic Acid

作　　者：胡永红[1] 罗辉[1] 陈武领[1] 邹家庆[1] 欧阳平凯[1] 杨文革[2] 

机构地区：[1]南京化工大学生物工程与科学系,南京210009 [2]南京药物研究所,南京210009

出　　处：《分析化学》1996年第6期677-680,共4页Chinese Journal of Analytical Chemistry

摘　　要：本文建立了简单、迅速地利用变色酸光度测定海因的方法。实验中将0．2％的变色酸浓硫酸显色剂加入试样，在150℃下加热4min形成玫瑰海因，再于549nm处测定其吸收值。该法检测海因含量准确可靠，测定范围在0～25mg／L之间具有良好的线性关系，相关系数为0．9998；回收率为96．8％～101．1％，且不受海因经乙内酰脲酶转化生成甘氨酸的干扰，具有良好的稳定性和重复性。A simple and rapid method for the spectrophotometric determination of hydantoin with chromotropic acid has been reported. In experiment, an 0. 2% indicating solution of chromotropic acid in concentrated sulfuric acid is added to the sample solution and the mixture is heated at 150℃ for 4 min to form roseolic hydantoin, then the absorbance of the solution is measured at 549 nm. The results obtained are accurate and reliable. The calibration curve is linear in the range of 0~25 mg/L. The correlation coefficient and the recovery are 0. 9998, 96. 8% ~101. 1 % respectively. The determination can effectively avoid the interference of glycine which is produced by hydantoinase from hydantoin and also has good reproducibility.

关 键 词：变色酸 分光光度法 咪唑烷二酮 

分 类 号：O626.23[理学—有机化学] O657.32[理学—化学]