反相高效液相色谱法测定嘌呤、嘧啶类化合物  

Separation of Purine and Pyrimidine Compounds by Reversed-Phase High Performance Liquid Chromatography

作　　者：刘绮萍[1] 瞿萍[1] 施玉芳 王明芝[1] 刘锦春[1] 

机构地区：[1]武汉大学化学系

出　　处：《色谱》1996年第4期310-312,共3页Chinese Journal of Chromatography

摘　　要：用反相高效液相色谱法同时分离和测定了胞嘧啶、胸腺嘧啶、腺嘌呤、茶碱和咖啡因等５种结构相似的生物碱基。方法简便、快速，用于茶样中咖啡因的测定，标准加入回收率在９５．６９％～１０４．２０％之间。This paper reports the separation and determination of cytosine, thymine, adenine, theophylline,and caffeine by reversed-phase HPLC. The five bases with similar structures can be separated on Spherisorb C_(18)column at 35℃ with UV detection at 260nm. The mobile phase is acetonitrile-water-potassium dihydrogen phosphate-acetic acid (2:96. 9:1:0.1) flowing at a rate of 1. 1 mL/min. The method is simple and rapid, and has been used for analysis of caffeine in tea samples. The results are satisfactory and the recoveries with standard addition were in the range of 95. 69%-104. 20%.

关 键 词：高效液相色谱法 生物碱基 茶叶 嘌呤 嘧啶 

分 类 号：Q526.2[生物学—生物化学] O657.72[理学—分析化学]