间质性膀胱炎病人膀胱粘膜HLA-DR表达的研究  

Study on HLA-DR expression of interstitial cystitis

作　　者：王旭[1] 侯磊[1] 韩瑞发[2] 

机构地区：[1]北京航天总医院泌尿外科,100076 [2]天津医科大学第二医院,天津市泌尿外科研究所

出　　处：《中国现代医药杂志》2006年第4期28-30,共3页Modern Medicine Journal of China

摘　　要：目的对比间质性膀胱炎病人及正常人MHC-Ⅱ类抗原中HLA-DR分子在膀胱上皮组织及其淋巴细胞中的表达情况,以确定间质性膀胱炎病人是否存在自身免疫疾病方面的重要特征,即上皮细胞的HLA-DR过表达。对间质性膀胱炎疾病的发病机制,尤其是自身免疫机制进行初步的研究。方法间质性膀胱炎病人及正常人两组共22份膀胱组织标本(各11份),以小鼠抗人HLA-DR单克隆抗体作为一抗,羊抗小鼠IgG抗体-HRP多聚体作为二抗进行免疫组化染色。并对两组标本表达情况进行统计学分析。结果正常女性组膀胱组织标本中,未见明显膀胱上皮细胞的HLA-DR表达。而在间质性膀胱炎病人组,可见不同程度的HLA-DR过表达,两组结果分析,它们的差异有统计学意义。结论间质性膀胱炎的病因发病可能与膀胱粘膜的自身免疫反应有关。Objective To study the expression of HLA-DR molecules in the epithelium and lymphocytes cells in the submucose from 22 cases of interstitial cystitis and control groups. Methods Indirect immunofiuorescence was performed on the biopsies using murine MoAbs to human HLA class Ⅱ molecules HLA-DR. Results In all cases of interstitial cystitis showed surface expression of HLA-DR molecules in the epithelium and lymphocytes cells in the submucose. Statistical evaluation suggests that the abnormal urothelial HLA-DR expression in interstitial cystitis is greater than normal bladder.Conclusion The findings provide further evidence for the importance of imappropriate HLA-DR molecule expression in intecstitial cystitis suspected having an autoimmune pathogenesis and where cellular autoimmune mechanisms play a decisive role in the destrucion of the target cell-the bladder urothelium.

关 键 词：间质性膀胱炎 免疫组化 HLA—DR表达 自身免疫 

分 类 号：R694[医药卫生—泌尿科学]